Overview

  • Product name

    Anti-C1q antibody [JL-1]
    See all C1q primary antibodies
  • Description

    Mouse monoclonal [JL-1] to C1q
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, ELISA, Functional Studies, WB, IHC-Fr, IHC-FoFr, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Purified mouse C1q

  • Epitope

    The monoclonal antibody JL-1is reactive with the collagen-like region (CLR).
  • Positive control

    • Spleen and kidney tissue of wild-type mice.
  • General notes

    The antibody has been generated by immunization of C1q-/- C57BL/6 mice with purified mouse C1q.

Properties

Applications

Our Abpromise guarantee covers the use of ab71940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. JL-1 was administered to mice resulting in depletion of circulating C1q, glomerular deposition of C1q and induction of anti-C1q autoantibodies in susceptible mice. As a negative control an isotype matched monoclonal antibody was used.
WB 1/50. Predicted molecular weight: 26 kDa.
IHC-Fr 1/50.
IHC-FoFr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

Images

  • ab71940 staining C1q in rat spleen tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, blocked with 10% serum for 30 minutes at 24°C then incubated with ab71940 at a 1/50 dilution for 16 hours at 4°C. The seconday used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/1000 dilution.

    See Abreview

  • ab71940 staining C1q in mouse liver tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices, permeablized with 0.1 M PBS with 3% Triton X and blocked with 10% serum for 60 minutes at 24°C. The sample was incubated with primary antibody (1/100 in 0.3M PBST with 10% donkey serum) at 4°C for 24 hours. An Alexa Fluor® 488-conjugated donkey monoclonal (1/1000) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Bie B  et al. Activation of mGluR1 Mediates C1q-Dependent Microglial Phagocytosis of Glutamatergic Synapses in Alzheimer's Rodent Models. Mol Neurobiol N/A:N/A (2019). Read more (PubMed: 30652266) »
  • Zhu X  et al. All-trans retinoic acid protects mesenchymal stem cells from immune thrombocytopenia by regulating the complement-IL-1ß loop. Haematologica N/A:N/A (2019). Read more (PubMed: 30679324) »
See all 10 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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1-9 of 9 Abreviews

Application
ELISpot
Sample
Human Purified protein (Purified from serum)
Specification
Purified from serum

Abcam user community

Verified customer

Submitted Jun 25 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
Yes - 0.1 M PBS with 3% Triton X
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 12 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Mouse Tissue sections (spleen)
Specification
spleen
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 28 2014

Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Cell (Kelly Cell line)
Specification
Kelly Cell line
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 28 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10µg/mL · Temperature: 24°C
Sample
Human Tissue sections (Retina)
Specification
Retina
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 28 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Rat Cell (B50)
Specification
B50
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 28 2014

Application
Immunocytochemistry
Sample
Rat Cell (33B Cell line)
Specification
33B Cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Sep 26 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Kelly Neuroblastoma cell line)
Specification
Kelly Neuroblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Sep 26 2012

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spleen)
Specification
Spleen
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Aug 19 2011

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