Anti-C1QA antibody (ab155052)

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Western blot - Anti-C1QA antibody (ab155052)
  • Western blot - Anti-C1QA antibody (ab155052)
  • Immunocytochemistry/ Immunofluorescence - Anti-C1QA antibody (ab155052)

Key features and details

  • Rabbit polyclonal to C1QA
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-C1QA antibody
    See all C1QA primary antibodies

  • Description

    Rabbit polyclonal to C1QA

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 13-225 of Human C1QA (UniProt ID: P02745).

  • Positive control

    • HepG2 and mouse spleen whole cell lysates; HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

  • Involvement in disease

    Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.

  • Sequence similarities

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.

  • Post-translational
    modifications

    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • C1qa antibody
      • C1QA_HUMAN antibody
      • Complement C1q subcomponent subunit A antibody
      • Complement component 1 q subcomponent A chain antibody
      • Complement component 1 q subcomponent alpha polypeptide antibody
      • Complement component C1q A chain antibody
      see all

    Images

    • Western blot - Anti-C1QA antibody (ab155052)
      Western blot - Anti-C1QA antibody (ab155052)
      Anti-C1QA antibody (ab155052) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

      Predicted band size: 26 kDa



      12% SDS-PAGE.
    • Western blot - Anti-C1QA antibody (ab155052)
      Western blot - Anti-C1QA antibody (ab155052)
      Anti-C1QA antibody (ab155052) at 1/1000 dilution + mouse spleen whole cell lysate at 50 µg

      Predicted band size: 26 kDa



      12% SDS-PAGE.
    • Immunocytochemistry/ Immunofluorescence - Anti-C1QA antibody (ab155052)
      Immunocytochemistry/ Immunofluorescence - Anti-C1QA antibody (ab155052)
      Immunofluorescent analysis of methanol-fixed HeLa cells labeling C1QA with ab155052 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

    References (1)

    Publishing research using ab155052? Please let us know so that we can cite the reference in this datasheet.

    ab155052 has been referenced in 1 publication.

    • Barrios AA  et al. Inefficient and abortive classical complement pathway activation by the calcium inositol hexakisphosphate component of the Echinococcus granulosus laminated layer. Immunobiology 224:710-719 (2019). PubMed: 31178241

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