Anti-C1QA antibody (ab155052)
Overview
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Product name
Anti-C1QA antibody
See all C1QA primary antibodies -
Description
Rabbit polyclonal to C1QA -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 13-225 of Human C1QA (UniProt ID: P02745).
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Positive control
- HepG2 and mouse spleen whole cell lysates; HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 26 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
Involvement in disease
Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsO-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 712 Human
- Entrez Gene: 12259 Mouse
- Omim: 120550 Human
- SwissProt: P02745 Human
- SwissProt: P98086 Mouse
- Unigene: 632379 Human
- Unigene: 439957 Mouse
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Alternative names
- C1qa antibody
- C1QA_HUMAN antibody
- Complement C1q subcomponent subunit A antibody
see all
Images
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Western blot - Anti-C1QA antibody (ab155052)Anti-C1QA antibody (ab155052) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS-PAGE. -
Western blot - Anti-C1QA antibody (ab155052)Anti-C1QA antibody (ab155052) at 1/1000 dilution + mouse spleen whole cell lysate at 50 µg
Predicted band size: 26 kDa
12% SDS-PAGE. -
Immunocytochemistry/ Immunofluorescence - Anti-C1QA antibody (ab155052)Immunofluorescent analysis of methanol-fixed HeLa cells labeling C1QA with ab155052 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Protocols
References
ab155052 has not yet been referenced specifically in any publications.
