Product nameAnti-C1QA antibody
See all C1QA primary antibodies
DescriptionRabbit polyclonal to C1QA
Tested applicationsSuitable for: Flow Cyt, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat
Synthetic peptide corresponding to Mouse C1QA aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Mouse blood plasma tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab64632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 29364839|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 26 kDa).|
FunctionC1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Involvement in diseaseDefects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
Sequence similaritiesContains 1 C1q domain.
Contains 1 collagen-like domain.
modificationsO-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
- Information by UniProt
- C1qa antibody
- C1QA_HUMAN antibody
- Complement C1q subcomponent subunit A antibody
Anti-C1QA antibody (ab64632) at 1 µg/ml + Plasma (Mouse) Tissue Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 26 kDa
Observed band size: 36 + 33 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
The 33 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Mouse Complement C1q subcomponent subunit A precursor. C1q also contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.