Recombinant Anti-C1QA antibody [EPR14634] (ab189922)
![Western blot - Anti-C1QA antibody [EPR14634] (ab189922)](https://www.abcam.com/ps/products/189/ab189922/Images/ab189922-225722-anti-c1qa-antibody-epr14634-western-blot.jpg "Western blot - Anti-C1QA antibody [EPR14634] (ab189922)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14634] to C1QA
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-C1QA antibody [EPR14634]
See all C1QA primary antibodies -
Description
Rabbit monoclonal [EPR14634] to C1QA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human plasma, Human serum, Human spleen, C6 and PC-12 lysates; Human and rat kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14634 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
|
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
Involvement in disease
Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsO-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 712 Human
- Entrez Gene: 298566 Rat
- Omim: 120550 Human
- SwissProt: P02745 Human
- SwissProt: P31720 Rat
- Unigene: 632379 Human
- Unigene: 105647 Rat
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Alternative names
- C1qa antibody
- C1QA_HUMAN antibody
- Complement C1q subcomponent subunit A antibody
see all
Images
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Western blot - Anti-C1QA antibody [EPR14634] (ab189922)All lanes : Anti-C1QA antibody [EPR14634] (ab189922) at 1/10000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human serum lysate
Lane 3 : Human spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa -
![Western blot - Anti-C1QA antibody [EPR14634] (ab189922)](https://www.abcam.com/ps/products/189/ab189922/Images/ab189922-225721-anti-c1qa-antibody-epr14634-western-blot.jpg)
Western blot - Anti-C1QA antibody [EPR14634] (ab189922)All lanes : Anti-C1QA antibody [EPR14634] (ab189922) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR14634] (ab189922)](https://www.abcam.com/ps/products/189/ab189922/Images/ab189922-225720-anti-c1qa-antibody-epr14634-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR14634] (ab189922)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling C1QA with ab189922 at 1/1000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR14634] (ab189922)](https://www.abcam.com/ps/products/189/ab189922/Images/ab189922-225719-anti-c1qa-antibody-epr14634-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR14634] (ab189922)Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling C1QA with ab189922 at 1/1000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-C1QA antibody [EPR14634] (ab189922)](https://www.abcam.com/ps/products/189/ab189922/Images/ab189922-5-benefits-of-recombinant-antibodies.png)
Anti-C1QA antibody [EPR14634] (ab189922)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (3)
ab189922 has been referenced in 3 publications.
- Datta D et al. Classical complement cascade initiating C1q protein within neurons in the aged rhesus macaque dorsolateral prefrontal cortex. J Neuroinflammation 17:8 (2020). PubMed: 31906973
- Wang Q et al. Urinary phosphate-containing nanoparticle contributes to inflammation and kidney injury in a salt-sensitive hypertension rat model. Commun Biol 3:575 (2020). PubMed: 33060834
- VanRyzin JW et al. Microglial Phagocytosis of Newborn Cells Is Induced by Endocannabinoids and Sculpts Sex Differences in Juvenile Rat Social Play. Neuron 102:435-449.e6 (2019). PubMed: 30827729
