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    c1qa-antibody-epr14634-ab189922.pdf

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Immunology Innate Immunity Complement Classical Pathway
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RecombinantRabMAb

Recombinant Anti-C1QA antibody [EPR14634] (ab189922)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (3)

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Western blot - Anti-C1QA antibody [EPR14634] (ab189922)
  • Western blot - Anti-C1QA antibody [EPR14634] (ab189922)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR14634] (ab189922)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR14634] (ab189922)
  • Anti-C1QA antibody [EPR14634] (ab189922)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14634] to C1QA
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-C1QA antibody [EPR14634]
    See all C1QA primary antibodies
  • Description

    Rabbit monoclonal [EPR14634] to C1QA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human plasma, Human serum, Human spleen, C6 and PC-12 lysates; Human and rat kidney tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14634
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-C1QA antibody [EPR14634] - BSA and Azide free (ab251007)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human C1QA protein (ab157982)
  • Related Products

    • Recombinant Human C1QA protein (ab157982)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab189922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • Involvement in disease

    Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • Sequence similarities

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications

    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02745 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 712 Human
    • Entrez Gene: 298566 Rat
    • Omim: 120550 Human
    • SwissProt: P02745 Human
    • SwissProt: P31720 Rat
    • Unigene: 632379 Human
    • Unigene: 105647 Rat
    • Alternative names

      • C1qa antibody
      • C1QA_HUMAN antibody
      • Complement C1q subcomponent subunit A antibody
      • Complement component 1 q subcomponent A chain antibody
      • Complement component 1 q subcomponent alpha polypeptide antibody
      • Complement component C1q A chain antibody
      see all

    Images

    • Western blot - Anti-C1QA antibody [EPR14634] (ab189922)
      Western blot - Anti-C1QA antibody [EPR14634] (ab189922)
      All lanes : Anti-C1QA antibody [EPR14634] (ab189922) at 1/10000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human serum lysate
      Lane 3 : Human spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 26 kDa
      Observed band size: 26 kDa

    • Western blot - Anti-C1QA antibody [EPR14634] (ab189922)
      Western blot - Anti-C1QA antibody [EPR14634] (ab189922)
      All lanes : Anti-C1QA antibody [EPR14634] (ab189922) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : PC-12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 26 kDa
      Observed band size: 26 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR14634] (ab189922)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR14634] (ab189922)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling C1QA with ab189922 at 1/1000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR14634] (ab189922)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR14634] (ab189922)

      Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling C1QA with ab189922 at 1/1000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-C1QA antibody [EPR14634] (ab189922)
      Anti-C1QA antibody [EPR14634] (ab189922)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (3)

    Publishing research using ab189922? Please let us know so that we can cite the reference in this datasheet.

    ab189922 has been referenced in 3 publications.

    • Datta D  et al. Classical complement cascade initiating C1q protein within neurons in the aged rhesus macaque dorsolateral prefrontal cortex. J Neuroinflammation 17:8 (2020). PubMed: 31906973
    • Wang Q  et al. Urinary phosphate-containing nanoparticle contributes to inflammation and kidney injury in a salt-sensitive hypertension rat model. Commun Biol 3:575 (2020). PubMed: 33060834
    • VanRyzin JW  et al. Microglial Phagocytosis of Newborn Cells Is Induced by Endocannabinoids and Sculpts Sex Differences in Juvenile Rat Social Play. Neuron 102:435-449.e6 (2019). PubMed: 30827729

    Customer reviews and Q&As

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