RecombinantRabMAb

Recombinant Anti-C1QA antibody [EPR2980Y] (ab76425)

Submit a review Submit a question References (1)
Western blot - Anti-C1QA antibody [EPR2980Y] (ab76425)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR2980Y] (ab76425)
  • Anti-C1QA antibody [EPR2980Y] (ab76425)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2980Y] to C1QA
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-C1QA antibody [EPR2980Y]
    See all C1QA primary antibodies

  • Description

    Rabbit monoclonal [EPR2980Y] to C1QA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human C1QA aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human plasma lysate; human tonsil tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR2980Y

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Flow Cyt
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Flow Cyt
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

    • Involvement in disease

      Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.

    • Sequence similarities

      Contains 1 C1q domain.
      Contains 1 collagen-like domain.

    • Post-translational
      modifications

      O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • C1qa antibody
        • C1QA_HUMAN antibody
        • Complement C1q subcomponent subunit A antibody
        • Complement component 1 q subcomponent A chain antibody
        • Complement component 1 q subcomponent alpha polypeptide antibody
        • Complement component C1q A chain antibody
        see all

      Images

      • Western blot - Anti-C1QA antibody [EPR2980Y] (ab76425)
        Western blot - Anti-C1QA antibody [EPR2980Y] (ab76425)
        Anti-C1QA antibody [EPR2980Y] (ab76425) at 1/1000 dilution + human plasma lysate at 10 µg

        Secondary
        goat anti-rabbit HRP at 1/1000 dilution

        Predicted band size: 26 kDa
        Observed band size: 26 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR2980Y] (ab76425)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR2980Y] (ab76425)

        ab76425 at 1/100 dilution staining C1q in human tonsils by Immunohistochemistry, Paraffin-embedded tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-C1QA antibody [EPR2980Y] (ab76425)
        Anti-C1QA antibody [EPR2980Y] (ab76425)

      References (1)

      Publishing research using ab76425? Please let us know so that we can cite the reference in this datasheet.

      ab76425 has been referenced in 1 publication.

      • Duggan SP  et al. siRNA Library Screening Identifies a Druggable Immune-Signature Driving Esophageal Adenocarcinoma Cell Growth. Cell Mol Gastroenterol Hepatol 5:569-590 (2018). PubMed: 29930979

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