Overview

  • Product name

    Anti-C1QA antibody [EPR2980Y]
    See all C1QA primary antibodies
  • Description

    Rabbit monoclonal [EPR2980Y] to C1QA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human C1QA aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human plasma lysate; human tonsil tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100 - 1/250.
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
    • Involvement in disease

      Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
    • Sequence similarities

      Contains 1 C1q domain.
      Contains 1 collagen-like domain.
    • Post-translational
      modifications

      O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • C1qa antibody
      • C1QA_HUMAN antibody
      • Complement C1q subcomponent subunit A antibody
      • Complement component 1 q subcomponent A chain antibody
      • Complement component 1 q subcomponent alpha polypeptide antibody
      • Complement component C1q A chain antibody
      see all

    Images

    • Anti-C1QA antibody [EPR2980Y] (ab76425) at 1/1000 dilution + human plasma lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size: 26 kDa
      Observed band size: 26 kDa

    • ab76425 at 1/100 dilution staining C1q in human tonsils by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Duggan SP  et al. siRNA Library Screening Identifies a Druggable Immune-Signature Driving Esophageal Adenocarcinoma Cell Growth. Cell Mol Gastroenterol Hepatol 5:569-590 (2018). Read more (PubMed: 29930979) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab76425.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up