Recombinant Anti-C1QA antibody [EPR2980Y] (ab76425)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-C1QA antibody [EPR2980Y]
See all C1QA primary antibodies -
Description
Rabbit monoclonal [EPR2980Y] to C1QA -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, Flow Cyt, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human C1QA aa 200-300 (C terminal). The exact sequence is proprietary.
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Positive control
- Human plasma lysate; human tonsil tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2980Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab76425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | 1/100 - 1/250. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). | |
| Flow Cyt | 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. |
Target
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Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
Involvement in disease
Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsO-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 712 Human
- Omim: 120550 Human
- SwissProt: P02745 Human
- Unigene: 632379 Human
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Alternative names
- C1qa antibody
- C1QA_HUMAN antibody
- Complement C1q subcomponent subunit A antibody
see all
Images
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![Western blot - Anti-C1QA antibody [EPR2980Y] (ab76425)](https://www.abcam.com/ps/products/76/ab76425/Images/ab76425-91843-anti-c1qa-antibody-epr2980y-western-blot.gif)
Western blot - Anti-C1QA antibody [EPR2980Y] (ab76425)Anti-C1QA antibody [EPR2980Y] (ab76425) at 1/1000 dilution + human plasma lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR2980Y] (ab76425)](https://www.abcam.com/ps/products/76/ab76425/Images/ab76425-91842-anti-c1qa-antibody-epr2980y-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR2980Y] (ab76425)ab76425 at 1/100 dilution staining C1q in human tonsils by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Duggan SP et al. siRNA Library Screening Identifies a Druggable Immune-Signature Driving Esophageal Adenocarcinoma Cell Growth. Cell Mol Gastroenterol Hepatol 5:569-590 (2018). Read more (PubMed: 29930979) »
