Recombinant Anti-C1QA antibody [EPR2980Y] - BSA and Azide free (ab247472)
![Western blot - Anti-C1QA antibody [EPR2980Y] - BSA and Azide free (ab247472)](https://www.abcam.com/ps/products/247/ab247472/Images/ab247472-402405-anti-c1qa-antibody-epr2980y-bsa-and-azide-free-western-blot.gif "Western blot - Anti-C1QA antibody [EPR2980Y] - BSA and Azide free (ab247472)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2980Y] to C1QA - BSA and Azide free
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-C1QA antibody [EPR2980Y] - BSA and Azide free
See all C1QA primary antibodies -
Description
Rabbit monoclonal [EPR2980Y] to C1QA - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human C1QA aa 200-300 (C terminal). The exact sequence is proprietary.
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General notes
ab247472 is the carrier-free version of ab76425 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247472 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR2980Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab247472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP conjugated or polymerized HRP secondary antibodies is recommended. Stronger signals have been found using the polymerized HRP secondary.
|
Target
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Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
Involvement in disease
Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsO-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 712 Human
- Omim: 120550 Human
- SwissProt: P02745 Human
- Unigene: 632379 Human
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Alternative names
- C1qa antibody
- C1QA_HUMAN antibody
- Complement C1q subcomponent subunit A antibody
see all
Images
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Western blot - Anti-C1QA antibody [EPR2980Y] - BSA and Azide free (ab247472)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR2980Y] - BSA and Azide free (ab247472)](https://www.abcam.com/ps/products/247/ab247472/Images/ab247472-1-anti-c1qa-antibody-epr2980y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR2980Y] - BSA and Azide free (ab247472) -
![Anti-C1QA antibody [EPR2980Y] - BSA and Azide free (ab247472)](https://www.abcam.com/ps/products/247/ab247472/Images/ab247472-3-benefits-of-recombinant-antibodies.png)
Anti-C1QA antibody [EPR2980Y] - BSA and Azide free (ab247472)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab247472 has not yet been referenced specifically in any publications.
