Recombinant
RabMAb

Recombinant Anti-C1QA antibody [EPR2980Y] - BSA and Azide free (ab247472)

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Overview

  • Product name

    Anti-C1QA antibody [EPR2980Y] - BSA and Azide free
    See all C1QA primary antibodies

  • Description

    Rabbit monoclonal [EPR2980Y] to C1QA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt, WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human C1QA aa 200-300 (C terminal). The exact sequence is proprietary.

  • General notes

    ab247472 is the carrier-free version of ab76425 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247472 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR2980Y

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab247472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP conjugated or polymerized HRP secondary antibodies is recommended. Stronger signals have been found using the polymerized HRP secondary. 

 
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

    • Involvement in disease

      Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.

    • Sequence similarities

      Contains 1 C1q domain.
      Contains 1 collagen-like domain.

    • Post-translational
      modifications

      O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • C1qa antibody
        • C1QA_HUMAN antibody
        • Complement C1q subcomponent subunit A antibody
        • Complement component 1 q subcomponent A chain antibody
        • Complement component 1 q subcomponent alpha polypeptide antibody
        • Complement component C1q A chain antibody
        see all

      References

      ab247472 has not yet been referenced specifically in any publications.

      Publishing research using ab247472? Please let us know so that we can cite the reference in this datasheet.

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