Recombinant Anti-C1r antibody [EPR14915] (ab185212)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14915] to C1r
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-C1r antibody [EPR14915]
See all C1r primary antibodies -
Description
Rabbit monoclonal [EPR14915] to C1r -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human plasma lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14915 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/40 - 1/60.
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).
This antibody may be used diluted 1:2000 (PMID: 31749804). |
Notes |
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IP
1/40 - 1/60. |
WB
1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa). This antibody may be used diluted 1:2000 (PMID: 31749804). |
Target
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Function
C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 1 EGF-like domain.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Post-translational
modificationsThe iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. - Information by UniProt
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Database links
- Entrez Gene: 715 Human
- Omim: 613785 Human
- SwissProt: P00736 Human
- Unigene: 524224 Human
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Alternative names
- C1 R antibody
- C1R antibody
- C1R_HUMAN antibody
see all
Images
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Anti-C1r antibody [EPR14915] (ab185212) at 1/5000 dilution + Human plasma lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 80 kDa -
Western blot analysis of immunoprecipitation pellet from Human plasma lysate immunoprecipitated using ab185212 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab185212 has been referenced in 1 publication.
- Gröbner R et al. C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome. Front Immunol 10:2537 (2019). WB ; Human . PubMed: 31749804