Overview

  • Product name

    Anti-C1r antibody [EPR14915]
    See all C1r primary antibodies
  • Description

    Rabbit monoclonal [EPR14915] to C1r
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human C1r aa 600-700. The exact sequence is proprietary.
    Database link: P00736

  • Positive control

    • Human plasma lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14915
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40 - 1/60.
WB 1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).

This antibody may be used diluted 1:2000 (PMID: 31749804).

Target

  • Function

    C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 1 EGF-like domain.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Post-translational
    modifications

    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1 R antibody
    • C1R antibody
    • C1R_HUMAN antibody
    • Complement C1r antibody
    • Complement C1r subcomponent antibody
    • Complement C1r subcomponent light chain antibody
    • Complement C1r subcomponent precursor antibody
    • Complement component 1 r subcomponent antibody
    • Complement component 1 subcomponent r antibody
    see all

Images

  • Anti-C1r antibody [EPR14915] (ab185212) at 1/5000 dilution + Human plasma lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

  • Western blot analysis of immunoprecipitation pellet from Human plasma lysate immunoprecipitated using ab185212 at 1/50 dilution (lane 1) or PBS control (lane 2).
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

This product has been referenced in:

  • Gröbner R  et al. C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome. Front Immunol 10:2537 (2019). WB ; Human . Read more (PubMed: 31749804) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (skin fibroblast)
Gel Running Conditions
Reduced Denaturing (10 % SDS gel)
Loading amount
20 µg
Specification
skin fibroblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Nov 30 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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