Product nameAnti-C1s antibody [EPR9066(B)]
See all C1s primary antibodies
DescriptionRabbit monoclonal [EPR9066(B)] to C1s
Tested applicationsSuitable for: Flow Cyt, IHC-P, WB, IPmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human C1s aa 1-100. The exact sequence is proprietary.
- Human liver tissue; A431, Human fetal kidney, plasma, platelet and serum lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 77 kDa.|
|IP||Use at an assay dependent concentration.|
FunctionC1s B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
Involvement in diseaseDefects in C1S are the cause of complement component C1s deficiency (C1SD) [MIM:613783]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 1 EGF-like domain.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains.
modificationsThe iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
- Information by UniProt
- Basic proline rich peptide IB 1 antibody
- C1 esterase antibody
- C1S antibody
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human liver tissue, labelling C1s using ab134943 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-C1s antibody [EPR9066(B)] (ab134943) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : A431 cell lysate
Lane 3 : Human platelet lysate
Lane 4 : Human plasma lysate
Lane 5 : Human serum lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 77 kDa
Western blot analysis on Immunoprecipitation pellet from human plasma lysate using anti-C1s RabMAb (ab134943).
This product has been referenced in:
- Chang IW et al. Complement Component 1, s Subcomponent Overexpression is an Independent Poor Prognostic Indicator in Patients with Urothelial Carcinomas of the Upper Urinary Tract and Urinary Bladder. J Cancer 7:1396-405 (2016). IHC . Read more (PubMed: 27471555) »