RecombinantRabMAb

Recombinant Anti-C1s antibody [EPR9066(B)] (ab134943)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1s antibody [EPR9066(B)] (ab134943)
  • Western blot - Anti-C1s antibody [EPR9066(B)] (ab134943)
  • Immunoprecipitation - Anti-C1s antibody [EPR9066(B)] (ab134943)
  • Anti-C1s antibody [EPR9066(B)] (ab134943)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9066(B)] to C1s
  • Suitable for: Flow Cyt, IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-C1s antibody [EPR9066(B)]
    See all C1s primary antibodies

  • Description

    Rabbit monoclonal [EPR9066(B)] to C1s

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human C1s aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Human liver tissue; A431, Human fetal kidney, plasma, platelet and serum lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9066(B)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
IP
Human
WB
Human
Application Abreviews Notes
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IP
Use at an assay dependent concentration.
Notes
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended.
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
IP
Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      C1s B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.

    • Involvement in disease

      Defects in C1S are the cause of complement component C1s deficiency (C1SD) [MIM:613783]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.

    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 2 CUB domains.
      Contains 1 EGF-like domain.
      Contains 1 peptidase S1 domain.
      Contains 2 Sushi (CCP/SCR) domains.

    • Post-translational
      modifications

      The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    • Information by UniProt

    • Database links

      • Alternative names

        • Basic proline rich peptide IB 1 antibody
        • C1 esterase antibody
        • C1S antibody
        • C1S_HUMAN antibody
        • Complement C1s subcomponent antibody
        • Complement C1s subcomponent heavy chain antibody
        • Complement C1s subcomponent light chain antibody
        • Complement component 1 s subcomponent antibody
        • Complement component 1 subcomponent s antibody
        • FLJ44757 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1s antibody [EPR9066(B)] (ab134943)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1s antibody [EPR9066(B)] (ab134943)

        Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human liver tissue, labelling C1s using ab134943 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-C1s antibody [EPR9066(B)] (ab134943)
        Western blot - Anti-C1s antibody [EPR9066(B)] (ab134943)
        All lanes : Anti-C1s antibody [EPR9066(B)] (ab134943) at 1/1000 dilution

        Lane 1 : Human fetal kidney lysate
        Lane 2 : A431 cell lysate
        Lane 3 : Human platelet lysate
        Lane 4 : Human plasma lysate
        Lane 5 : Human serum lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 77 kDa

      • Immunoprecipitation - Anti-C1s antibody [EPR9066(B)] (ab134943)
        Immunoprecipitation - Anti-C1s antibody [EPR9066(B)] (ab134943)
        Western blot analysis on Immunoprecipitation pellet from human plasma lysate using anti-C1s RabMAb (ab134943).
      • Anti-C1s antibody [EPR9066(B)] (ab134943)
        Anti-C1s antibody [EPR9066(B)] (ab134943)

      References (1)

      Publishing research using ab134943? Please let us know so that we can cite the reference in this datasheet.

      ab134943 has been referenced in 1 publication.

      • Chang IW  et al. Complement Component 1, s Subcomponent Overexpression is an Independent Poor Prognostic Indicator in Patients with Urothelial Carcinomas of the Upper Urinary Tract and Urinary Bladder. J Cancer 7:1396-405 (2016). IHC . PubMed: 27471555

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