Recombinant Anti-C1s antibody [EPR9066(B)] (ab134943)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-C1s antibody [EPR9066(B)]
See all C1s primary antibodies -
Description
Rabbit monoclonal [EPR9066(B)] to C1s -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WB, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human C1s aa 1-100. The exact sequence is proprietary.
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Positive control
- Human liver tissue; A431, Human fetal kidney, plasma, platelet and serum lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9066(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98°C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 77 kDa. | |
| IP | Use at an assay dependent concentration. |
Target
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Function
C1s B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4. -
Involvement in disease
Defects in C1S are the cause of complement component C1s deficiency (C1SD) [MIM:613783]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 1 EGF-like domain.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Post-translational
modificationsThe iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. - Information by UniProt
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Database links
- Entrez Gene: 716 Human
- Omim: 120580 Human
- SwissProt: P09871 Human
- Unigene: 458355 Human
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Alternative names
- Basic proline rich peptide IB 1 antibody
- C1 esterase antibody
- C1S antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1s antibody [EPR9066(B)] (ab134943)](https://www.abcam.com/ps/products/134/ab134943/Images/ab134943-149141-anti-c1s-antibody-epr9066b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1s antibody [EPR9066(B)] (ab134943)Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human liver tissue, labelling C1s using ab134943 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-C1s antibody [EPR9066(B)] (ab134943)](https://www.abcam.com/ps/products/134/ab134943/Images/ab134943-149142-anti-c1s-antibody-epr9066b-western-blot.jpg)
Western blot - Anti-C1s antibody [EPR9066(B)] (ab134943)All lanes : Anti-C1s antibody [EPR9066(B)] (ab134943) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : A431 cell lysate
Lane 3 : Human platelet lysate
Lane 4 : Human plasma lysate
Lane 5 : Human serum lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 77 kDa -
![Immunoprecipitation - Anti-C1s antibody [EPR9066(B)] (ab134943)](https://www.abcam.com/ps/products/134/ab134943/Images/ab134943-149140-anti-c1s-antibody-epr9066b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-C1s antibody [EPR9066(B)] (ab134943)Western blot analysis on Immunoprecipitation pellet from human plasma lysate using anti-C1s RabMAb (ab134943).
Protocols
References
This product has been referenced in:
- Chang IW et al. Complement Component 1, s Subcomponent Overexpression is an Independent Poor Prognostic Indicator in Patients with Urothelial Carcinomas of the Upper Urinary Tract and Urinary Bladder. J Cancer 7:1396-405 (2016). IHC . Read more (PubMed: 27471555) »
