Overview

  • Product name

    Anti-C2 antibody [5C3]
    See all C2 primary antibodies
  • Description

    Mouse monoclonal [5C3] to C2
  • Host species

    Mouse
  • Specificity

    This antibody reacts with C2 and a subfraction of C2 believed to be C2b
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native complement component C2 protein isolated from human plasma.

  • Epitope

    Epitope specificity differs from that of ab17452 as determined by inhibition ELISA.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    5C3
  • Myeloma

    x63-Ag8.653
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/12000.
WB Use at an assay dependent dilution. Predicted molecular weight: 102 kDa.

Target

  • Function

    Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
  • Involvement in disease

    Defects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 3 Sushi (CCP/SCR) domains.
    Contains 1 VWFA domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARMD14 antibody
    • C2 antibody
    • C3/C5 convertase antibody
    • CO2 antibody
    • CO2_HUMAN antibody
    • Complement C2 antibody
    • Complement C2a fragment antibody
    • complement component 2 antibody
    • DKFZp779M0311 antibody
    see all

References

This product has been referenced in:

  • Rorbach J  et al. PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human mitochondria. Nucleic Acids Res : (2011). WB ; Human . Read more (PubMed: 21666256) »
  • Stenbaek EI  et al. Human complement component C2: production and characterization of polyclonal and monoclonal antibodies against C2. Mol Immunol 23:879-86 (1986). Read more (PubMed: 3796629) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Gracias por contactarnos. Te pido disculpas por la espera.

ab17451 reacciona con la proteína C2 humana en sus formas nativa y desnaturalizada. En WB el anticuerpo reconoce C2 y una fracción de la misma, probablemente C2b, ambas bajo condiciones reductoras y no reductoras. En ELISA el anticuerpo presenta una elevada especificidad por C2 en los ensayos realizados cubriendo la placa con el anticuerpo.

No se ha testado si el anticuerpo reacciona con el fragmento C2b en su forma purificada.

Espero haberte ayudado. Para cualquier otra consulta o sugerencia, por favor no dudes en volvernos a contactar.

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Question
Answer

Thank you for your enquiry. I received the following information from the originator of these two antibodies: "The protocol we used to test these antibodies was a direct ELISA with a coat of C2, a layer with ab17451 (1:6500) or ab17452 (1:500), and detection was done with DAKO P260." I realize that these dilutions are different from what we had on the online datasheets and I have updated the datasheets to reflect this information. Please contact us again if you have any additional questions.

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