Overview

  • Product name

    Anti-C2 antibody [6E3]
    See all C2 primary antibodies
  • Description

    Mouse monoclonal [6E3] to C2
  • Host species

    Mouse
  • Specificity

    Strong reaction is seen with human serum/plasma and no reaction is seen with serum from patients deficient in C2. ab17452 reacts with C2 and a subfraction of C2 believed to be C2b.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native complement component C2 protein, isolated from human plasma.

  • Epitope

    Epitope specificity differs from that of ab17451 as determined by inhibition ELISA.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    6E3
  • Myeloma

    x63-Ag8.653
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/500.
    WB: 1/250. Predicted molecular weight: 102 kDa. ab17452 reacts with C2 under reducing and non-reducing conditions.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
    • Involvement in disease

      Defects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections.
    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 1 peptidase S1 domain.
      Contains 3 Sushi (CCP/SCR) domains.
      Contains 1 VWFA domain.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • ARMD14 antibody
      • C2 antibody
      • C3/C5 convertase antibody
      • CO2 antibody
      • CO2_HUMAN antibody
      • Complement C2 antibody
      • Complement C2a fragment antibody
      • complement component 2 antibody
      • DKFZp779M0311 antibody
      see all

    References

    This product has been referenced in:

    • Martini PG  et al. Recombinant human complement component C2 produced in a human cell line restores the classical complement pathway activity in-vitro: an alternative treatment for C2 deficiency diseases. BMC Immunol 11:43 (2010). Sandwich ELISA ; Human . Read more (PubMed: 20727163) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Question
    Answer

    Thank you for your enquiry. I received the following information from the originator of these two antibodies: "The protocol we used to test these antibodies was a direct ELISA with a coat of C2, a layer with ab17451 (1:6500) or ab17452 (1:500), and detection was done with DAKO P260." I realize that these dilutions are different from what we had on the online datasheets and I have updated the datasheets to reflect this information. Please contact us again if you have any additional questions.

    Read More

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