Key features and details
- Rabbit polyclonal to C20orf112/NOL4L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C20orf112/NOL4L antibody
DescriptionRabbit polyclonal to C20orf112/NOL4L
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Orangutan
Recombinant fragment corresponding to Human C20orf112/NOL4L aa 101-300.
LGTASYPSDGCGADGLRSRVKYGVKTTPESPPYSSGSYDSIKTEVSGCPE DLTVGRAPTADDDDDDHDDHEDNDKMNDSEGMDPERLKAFNMFVRLFVDE NLDRMVPISKQPKEKIQAIIESCSRQFPEFQERARKRIRTYLKSCRRMKK NGMEMTRPTPPHLTSAMAENILAAACESETRKAAKRMRLEIYQSSQDEPI
Database link: Q96MY1
- WB: PC-3, A549, HepG2 and K562 whole cell lysates. IHC-P: Human thyroid, testis, small intestine and adrenal gland tissues. ICC/IF: HeLa cells.
This product was previously labelled as C20orf112
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 47 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
- Information by UniProt
- C20orf112 antibody
- C20orf113 antibody
- Chromosome 20 open reading frame 112 antibody
All lanes : Anti-C20orf112/NOL4L antibody (ab237758) at 1/500 dilution
Lane 1 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa
Paraffin-embedded human thyroid tissue stained for C20orf112/NOL4L with ab237758 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for C20orf112/NOL4L with ab237758 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for C20orf112/NOL4L with ab237758 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for C20orf112/NOL4L with ab237758 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for C20orf112/NOL4L (green) using ab237758 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237758 has not yet been referenced specifically in any publications.