Overview

  • Product name

    Anti-C20orf118 antibody
  • Description

    Rabbit polyclonal to C20orf118
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 51-128 of Human C20orf118 (UniProt A0PJX2).

  • Positive control

    • Human rectum tissue; RT4 lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500. Predicted molecular weight: 24 kDa.

Target

  • Relevance

    Belongs to the OXR1 family. Contains 1 TLD domain.
  • Database links

  • Alternative names

    • Chromosome 20 open reading frame 118 antibody
    • dJ132F21.2 antibody
    • RP1-132F21.2 antibody
    • Uncharacterized protein C20orf118 antibody

Images

  • Immunohistochemical analysis of Paraffin-embedded Human rectum tissue labeling C20orf118 with ab150987 at 1/50 dilution.
  • All lanes : Anti-C20orf118 antibody (ab150987) at 1/250 dilution

    Lane 1 : RT4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Predicted band size: 24 kDa

References

ab150987 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

The immunogen of ab150987 correspond to aa 51-128 of human C20orf118 and is 87% homologous to mouse sequence. This sequence shares the amino acids of TLD sequence of mouse so it is more likely that the antibody will detect the protein.

http://web.expasy.org/cgi-bin/blast/blast.pl?action=HTML&sequence=EASQVLSASEIRQLSFHFPPRVTGHPWSLVFCTSRDGFSLQSLYRRMEGCSGPVLLVLRDQDGQIFGAFSSSAIRLSK#A10

ab123931; this product was never tested with mouse samples. In case you are interested in trying this antibody then I would encourage you to participate in our 100% Abtrial discount programme - a free product can be claimed in exchange of review for this antibody. Please contact me for code if you accept our offer.

More details about the offer can be found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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