Key features and details
- Rabbit polyclonal to C20orf27
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C20orf27 antibody
DescriptionRabbit polyclonal to C20orf27
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- Human stomach tissue
General notesThe antibody solution should be gently mixed before use.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab150991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 19 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
- Chromosome 20 open reading frame 27 antibody
- UPF0687 protein C20orf27 antibody
Immunofluorescent staining of human cell line MCF7 shows positivity in cytoplasm & nucleus but excluded from the nucleoli.
Anti-C20orf27 antibody (ab150991) at 1/500 dilution + HEK293T whole cell lysate at 20 µg
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time: 2 minutes
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling C20orf27 with ab150991 at 1/50 dilution.
ab150991 has not yet been referenced specifically in any publications.