Anti-C20orf77 antibody (ab137246)
Key features and details
- Rabbit polyclonal to C20orf77
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-C20orf77 antibody -
Description
Rabbit polyclonal to C20orf77 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human C20orf77 aa 250-350.
Database link: Q9NQG5 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and TCMK cell lysates. IP: HeLa cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab137246 was affinity purified using an epitope specific to C20orf77 immobilized on solid support . -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/1000. Predicted molecular weight: 37 kDa.
|
|
IP |
Use at 2-10 µg/mg of lysate.
|
Notes |
---|
WB
1/2000 - 1/1000. Predicted molecular weight: 37 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
-
Relevance
C20orf77 interacts with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. It is a transcriptional regulator which enhances expression of CCND1. It promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. It also revents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 58490 Human
- Entrez Gene: 70470 Mouse
- Entrez Gene: 311591 Rat
- Omim: 614694 Human
- SwissProt: Q9NQG5 Human
- SwissProt: Q9CSU0 Mouse
-
Alternative names
- C20orf77 antibody
- Cell cycle related and expression elevated protein in tumor antibody
- Chromosome 20 open reading frame 77 antibody
see all
Images
-
All lanes : Anti-C20orf77 antibody (ab137246) at 0.1 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 37 kDa
Exposure time: 10 secondsWestern blot analysis labeling C20orf77 with ab137246 at 0.1 ug/ml.
-
Lane 1: ab137246Lane 2: Control IgGImmunoprecipitation of C20orf77 from whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) of HeLa cells prepared using NETN lysis buffer. ab137246 was used for IP at 3 µg per reaction. For blotting immunoprecipitated RPRD1B, ab137246 was used at 1 µg/ml. Detection was by chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab137246 has not yet been referenced specifically in any publications.