Overview

  • Product name

  • Description

    Rabbit polyclonal to C20orf77
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 276-326 of Human C20orf77 (NP_067038.1).

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysate (ab7899), mouse NIH3T3 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab137246 was affinity purified using an epitope specific to C20orf77 immobilized on solid support .
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/1000. Predicted molecular weight: 37 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    C20orf77 interacts with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. It is a transcriptional regulator which enhances expression of CCND1. It promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. It also revents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • C20orf77 antibody
    • Cell cycle related and expression elevated protein in tumor antibody
    • Chromosome 20 open reading frame 77 antibody
    • CREPT antibody
    • dJ1057B20.2 antibody
    • DKFZp434P0735 antibody
    • FLJ44520 antibody
    • Hypothetical protein LOC58490 antibody
    • NET60 antibody
    • Regulation of nuclear pre mRNA domain containing 1B antibody
    • Regulation of nuclear pre mRNA domain containing protein 1B antibody
    • Uncharacterized protein C20orf77 antibody
    see all

Images

  • All lanes : Anti-C20orf77 antibody (ab137246) at 0.1 µg/ml

    Lane 1 : HeLa whole cell
    lysate at 50 µg
    Lane 2 : HeLa whole cell
    lysate at 15 µg
    Lane 3 : 293T whole cell
    lysate at 50 µg
    Lane 4 : Jurkat whole cell
    lysate at 50 µg
    Lane 5 : mouse NIH3T3 cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 37 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation with ab137246 at 6µg/mg lysate. 1 mg of HeLa whole cell lysate used for IP (20% of IP loaded). Western blot labelling C20orf77 with ab372461 at 1 µg/ml. Detected by chemiluminescence with exposure time of 10 seconds.

References

ab137246 has not yet been referenced specifically in any publications.

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