Key features and details
- Rabbit polyclonal to C20orf77
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-C20orf77 antibody
See all C20orf77 primary antibodies
DescriptionRabbit polyclonal to C20orf77
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Recombinant fragment corresponding to a region within amino acids 75-315 of Human C20orf77 (UniProt ID: Q9NQG5).
- 293T, HeLa and HepG2 whole cell lysates; SkHep1 and C2C12 xenograft tissues.
General notesKeep as concentrated solution.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 37 kDa.|
|IHC-P||1/100 - 1/1000.|
RelevanceC20orf77 interacts with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. It is a transcriptional regulator which enhances expression of CCND1. It promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. It also revents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.
- C20orf77 antibody
- Cell cycle related and expression elevated protein in tumor antibody
- Chromosome 20 open reading frame 77 antibody
All lanes : Anti-C20orf77 antibody (ab154910) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
10% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human SkHep1 xenograft tissue labeling C20orf77 with ab154910 at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded Mouse C2C12 xenograft tissue labeling C20orf77 with ab154910 at 1/500 dilution.
ab154910 has not yet been referenced specifically in any publications.