Recombinant
RabMAb

Anti-C21orf33 antibody [EPR13213] (ab181366)

Overview

  • Product name
    Anti-C21orf33 antibody [EPR13213]
    See all C21orf33 primary antibodies
  • Description
    Rabbit monoclonal [EPR13213] to C21orf33
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human C21orf33. The exact sequence is proprietary.
    Database link: P30042

  • Positive control
    • A549, HeLa, HepG2 and K562 cell lysates, Human heart tissue, Human liver tissue and HepG2 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P 1/50 - 1/100.

Perform heat mediated antigen retrieval, heat up to 98 degrees C, below boiling, and then let cool for 10-20 min, before commencing with IHC staining.

ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Relevance
      C21orf33 encodes a 268 amino acid protein which shares significant homology with Escherichia coli anti-sigma cross-reacting protein (SCRP-27A/SCRP27) and zebrafish Es1 protein. The specific function of the C21orf33 protein is not known.
    • Cellular localization
      Mitochondrial
    • Database links
    • Alternative names
      • Chromosome 21 open reading frame 33 antibody
      • D21S2048E antibody
      • ES 1 antibody
      • ES1 antibody
      • Es1 protein antibody
      • ES1 protein homolog mitochondrial antibody
      • GT335 antibody
      • HES 1 antibody
      • HES1 antibody
      • Human HES1 protein homolog to E.coli and zebrafish ES1 protein antibody
      • Keio novel protein I antibody
      • KNP I antibody
      • KNPH antibody
      • KNPI antibody
      • Protein GT335 antibody
      • Protein KNP I antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate (lane 1) or 1XPBS (lane 2) labeling C21orf33 using ab181366 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Immunofluorescent staining of HepG2 cells labeling C21orf33 using ab181366 at a 1/100 dilution (red) with DAPI nuclear staining (blue).

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling C21orf33 using ab181366 at a 1/50 dilution

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling C21orf33 using ab181366 at a 1/50 dilution.

    • All lanes : Anti-C21orf33 antibody [EPR13213] (ab181366) at 1/5000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 25 kDa

    References

    ab181366 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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