Key features and details
- Rabbit polyclonal to C21orf66
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C21orf66 antibody
DescriptionRabbit polyclonal to C21orf66
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176590 was affinity purified using an epitope specific to C21orf66 immobilized on solid support.
Our Abpromise guarantee covers the use of ab176590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 105 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPossible transcription factor.
Sequence similaritiesBelongs to the GCF family.
- Information by UniProt
- BM 020 antibody
- BM020 antibody
- Chromosome 21 open reading frame 66 antibody
Detection of C21orf66 by Western Blot of Immunprecipitate.
ab176590 at 1µg/ml labeling C21orf66 in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded) immunoprecipitated using ab176590 at 6µg/mg lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
All lanes : Anti-C21orf66 antibody (ab176590) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 30 seconds
ab176590 has not yet been referenced specifically in any publications.