Overview

  • Product name

  • Description

    Goat polyclonal to C22orf28
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human C22orf28 aa 201-214 (internal sequence) (Cysteine residue).
    Sequence:

    QADPNKVSARAKKR


    Database link: NP_055121.1

  • Positive control

    • WB: HeLa, Jurkat and K562 lysates. IHC-P: Human testis tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab118290 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Staining of Human Testis by ab118290 (5μg/mL) in paraffin embedded tissue following stemaed antigen retieval with citrat buffer pH6, AP-Staining this shows nuclear and vicinity staining in spermatogonia.

  • Anti-C22orf28 antibody (ab118290) at 0.3 µg/ml + HeLa lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    Primary incubation was 1 hour.

References

ab118290 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
5 µg
Specification
HeLa Cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 28 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
40 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 02 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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