Overview

  • Product name

  • Description

    Rabbit polyclonal to C22orf28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 107-156 (PEAVVSPGGV GFDINCGVRL LRTNLDESDV QPVKEQLAQA MFDHIPVGVG) of Human C22orf28 (NP_055121).

  • Positive control

    • HepG2 whole cell lysate (ab7900).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bronchial epithelial tissue labelling C22orf28 with ab98231 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - C22orf28. Right - Merge.
  • Western blot analysis of Jurkat cell lysate labeling C22orf28 with ab98231 at 1.0µg/ml.
  • Western blot analysis of Human fetal lung tissue lysate labeling C22orf28 with ab98231 at 1.0µg/ml.
  • Western blot analysis of HeLa cell lysate labeling C22orf28 with ab98231 at 1.0µg/ml.
  • Anti-C22orf28 antibody (ab98231) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size: 55 kDa

References

This product has been referenced in:

  • Moore MJ  et al. miRNA-target chimeras reveal miRNA 3'-end pairing as a major determinant of Argonaute target specificity. Nat Commun 6:8864 (2015). WB . Read more (PubMed: 26602609) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
40 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 02 2013

Answer

Thank you for your answer and sending the bovine sequence. Indeed, there is a 100% alingment with the immunogen and the chances are therefore very high that the antibody will crossreact. I am thus sending you here the testing discount code. Please see here the alignment results:

http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120828-161852-0516-45203912-oy

DISCOUNT CODE: xx
Expiration date: xx

I am very pleased to hear you would like to accept our offer and testab98231 in bovine cells. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forbovine cellsand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in ab98231.

Unfortunately we do not have any trial vials of our products. However if you would like to test this antibody, we can make you an interesting testing offer - please see below.

Do have by any chance the bovineC22orf28 protein sequence? I could not find it on UniProt. Ifyou have thebovine protein sequence,we could make an alignement with the immunogen sequence of ab98231 and make a prediction in regards to the crossreaction.

If there is a reasonable expectation of crossreaction,I can offer a discount off a future purchase if you buy ab98231 now, test it inbovine cellsand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and testab98231 in bovine cells. I will then send a discount code. This code must be issued before purchasing ab98231 so please wait for my reply before ordering.

2. Purchase ab98231 either by phone, fax, or online (www.abcam.com).

3. Test it in bovine cells.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifab98231 turns out to be unsuitable for bovine cells, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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