Product nameAnti-C2b antibody
DescriptionRabbit polyclonal to C2b
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
- This antibody gave a positive signal in Mouse liver tissue lysate.
This antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesThis antibody specifically recognises the C2b fragment of C2 (amino acids 21 - 243)
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab66999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 84 kDa).|
RelevanceComponent C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
- C2 antibody
- C2b antibody
- CO2 antibody
Anti-C2b antibody (ab66999) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Mouse Complement C2 precursor (C2b) contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The 97 kDa band observed is also comparable to the molecular weight seen with other commercially available antibodies to C2b.
ab66999 has not yet been referenced specifically in any publications.