Product nameC2C12 nuclear extract lysate
See all C2C12 lysates
General notesCell line: C2C12 (Mouse myoblast).
We recommend aliquoting the extracts into single use fractions and then storing.
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 7.90
Constituents: 0.75% Potassium chloride, 0.0087% PMSF, 0.0095% Magnesium chloride, 0.0077% DTT, 0.476% HEPES, 20% Glycerol
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BackgroundC2C12 cells were originally obtained by Yaffe and Saxel (1977) through selective serial passage of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush injury. These cells were shown to be capable of differentiation. C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins and the androhen receptor (AR).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab14640 has not yet been referenced specifically in any publications.