Anti-C2orf18 antibody - C-terminal (ab224147)
Key features and details
- Rabbit polyclonal to C2orf18 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C2orf18 antibody - C-terminal -
Description
Rabbit polyclonal to C2orf18 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Recombinant fragment corresponding to Human C2orf18 aa 335-371 (C terminal).
Sequence:GLHRPLLGRLSRGRPLAEESEQERLLGGTRTPINDAS
Database link: Q8N357 -
Positive control
- WB: C2orf18 overexpression HEK-293T cell lysate. IHC-P: Human cerebral cortex tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab224147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 40 kDa. | |
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
The exact function of C2orf18 remains unknown. It contains 1 DUF6 domain. -
Cellular localization
Membrane; Multi pass membrane protein -
Database links
- Entrez Gene: 54978 Human
- SwissProt: Q8N357 Human
- Unigene: 516034 Human
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Alternative names
- ANT2 binding protein antibody
- ANT2BP antibody
- Chromosome 2 open reading frame 18 antibody
- FLJ20555 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C2orf18 antibody - C-terminal (ab224147)
Paraffin-embedded human cerebral cortex tissue stained for C2orf18 using ab224147 at 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-C2orf18 antibody - C-terminal (ab224147)All lanes : Anti-C2orf18 antibody - C-terminal (ab224147) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : C2orf18 overexpression HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Developed using the ECL technique.
Predicted band size: 40 kDa
Protocols
Datasheets and documents
References (0)
ab224147 has not yet been referenced specifically in any publications.
