Anti-C2orf18 antibody - C-terminal (ab224147)

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Overview

  • Product name

    Anti-C2orf18 antibody - C-terminal
    See all C2orf18 primary antibodies

  • Description

    Rabbit polyclonal to C2orf18 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan

  • Immunogen

    Recombinant fragment corresponding to Human C2orf18 aa 335-371 (C terminal).
    Sequence:

    GLHRPLLGRLSRGRPLAEESEQERLLGGTRTPINDAS


    Database link: Q8N357

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: C2orf18 overexpression HEK-293T cell lysate. IHC-P: Human cerebral cortex tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/250. Predicted molecular weight: 40 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    The exact function of C2orf18 remains unknown. It contains 1 DUF6 domain.

  • Cellular localization

    Membrane; Multi pass membrane protein

  • Database links

    • Alternative names

      • ANT2 binding protein antibody
      • ANT2BP antibody
      • Chromosome 2 open reading frame 18 antibody
      • FLJ20555 antibody

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C2orf18 antibody - C-terminal (ab224147)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C2orf18 antibody - C-terminal (ab224147)

      Paraffin-embedded human cerebral cortex tissue stained for C2orf18 using ab224147 at 1/200 dilution in immunohistochemical analysis.

    • Western blot - Anti-C2orf18 antibody - C-terminal (ab224147)
      Western blot - Anti-C2orf18 antibody - C-terminal (ab224147)
      All lanes : Anti-C2orf18 antibody - C-terminal (ab224147) at 1/100 dilution

      Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
      Lane 2 : C2orf18 overexpression HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

      Developed using the ECL technique.

      Predicted band size: 40 kDa

    References

    ab224147 has not yet been referenced specifically in any publications.

    Publishing research using ab224147? Please let us know so that we can cite the reference in this datasheet.

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