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    c3-antibody-11h9-ab11862.pdf

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Immunology Innate Immunity Complement Classical Pathway
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Anti-C3 antibody [11H9] (ab11862)

  • Datasheet
  • SDS
Reviews (3)Q&A (9)References (29)

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Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody [11H9] (ab11862)

    Key features and details

    • Rat monoclonal [11H9] to C3
    • Suitable for: ICC/IF
    • Reacts with: Mouse
    • Isotype: IgG2a

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    Overview

    • Product name

      Anti-C3 antibody [11H9]
      See all C3 primary antibodies
    • Description

      Rat monoclonal [11H9] to C3
    • Host species

      Rat
    • Specificity

      This antibody recognizes both intact C3 and its cleaved products C3b, iC3b, C3d and C3dg. The mature protein C3 has a molecular weight of approximately 190 kDa. The complement factor C3 consists of an alpha- and a beta-chain, linked by disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylotoxin and generating C3b (alpha chain and beta chain). C3b has a molecular weight of approximately 185 kDa. C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. iC3b has a molecular weight of approximately 182 kDa. Does not cross react with C4.
    • Tested Applications & Species

      Application Species
      ICC/IF
      Mouse
      See all applications and species data
    • Immunogen

      C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1).

    • General notes

      In response to recent customer complaints for IHC-P with paraffin embedded sections we no longer guarantee this application.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituents: PBS, 0.1% BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      0.2 µm filtered
    • Clonality

      Monoclonal
    • Clone number

      11H9
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Innate Immunity
      • Complement
      • Classical Pathway
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker

    Associated products

    • Alternative Versions

      • Anti-C3b / iC3b antibody [7C12] (ab231078)
      • Anti-C3b / iC3b antibody [1H8] (ab231080)
    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Isotype control

      • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
    • Recombinant Protein

      • Recombinant Human C3 protein (ab157985)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab11862 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ICC/IF
    Mouse
    Application Abreviews Notes
    ICC/IF
    Use at an assay dependent concentration.
    Notes
    ICC/IF
    Use at an assay dependent concentration.

    Target

    • Function

      C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
      Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • Tissue specificity

      Plasma.
    • Involvement in disease

      Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
      Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
      Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
    • Sequence similarities

      Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Post-translational
      modifications

      C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P01024 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12266 Mouse
      • SwissProt: P01027 Mouse
      • Unigene: 19131 Mouse
      • Alternative names

        • Acylation stimulating protein cleavage product antibody
        • AHUS5 antibody
        • ARMD9 antibody
        • ASP antibody
        • C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
        • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
        • C3 antibody
        • c3 complement antibody
        • C3adesArg antibody
        • CO3_HUMAN antibody
        • Complement C3 alpha chain antibody
        • Complement C3 antibody
        • Complement C3b alpha' chain antibody
        • Complement C3c alpha' chain fragment 1 antibody
        • Complement C3c alpha' chain fragment 2 antibody
        • Complement C3c alpha'' chain fragment 2 antibody
        • Complement C3d fragment antibody
        • Complement C3dg fragment antibody
        • Complement C3f fragment antibody
        • Complement C3g fragment antibody
        • Complement component 3 antibody
        • Complement factor 3 antibody
        • CPAMD1 antibody
        • HEL S 62p antibody
        • omplement C3 beta chain antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody [11H9] (ab11862)
        Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody [11H9] (ab11862)
        ab11862 staining C3 in murine kidney cells by Immunocytochemistry/ Immunofluorescence.
        C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Glomerular staining pattern.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (29)

      Publishing research using ab11862? Please let us know so that we can cite the reference in this datasheet.

      ab11862 has been referenced in 29 publications.

      • Eder N  et al. YAP1/TAZ drives ependymoma-like tumour formation in mice. Nat Commun 11:2380 (2020). PubMed: 32404936
      • Li X  et al. Deficiency of Mouse FHR-1 Homolog, FHR-E, Accelerates Sepsis, and Acute Kidney Injury Through Enhancing the LPS-Induced Alternative Complement Pathway. Front Immunol 11:1123 (2020). PubMed: 32636836
      • Wehbi B  et al. Mesangial Deposition Can Strongly Involve Innate-Like IgA Molecules Lacking Affinity Maturation. J Am Soc Nephrol 30:1238-1249 (2019). PubMed: 31227634
      • Ugarte-Berzal E  et al. MMP-9/Gelatinase B Degrades Immune Complexes in Systemic Lupus Erythematosus. Front Immunol 10:538 (2019). PubMed: 30967870
      • Illouz T  et al. Restoring microglial and astroglial homeostasis using DNA immunization in a Down Syndrome mouse model. Brain Behav Immun 75:163-180 (2019). PubMed: 30389461
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 12 Abreviews or Q&A

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-C3 antibody [11H9]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Mouse Tissue sections (Adult brain)
      Antigen retrieval step
      None
      Permeabilization
      Yes - Triton 0.3%
      Specification
      Adult brain
      Blocking step
      BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More

      Dr. Sébastien Brot

      Verified customer

      Submitted Jul 09 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-C3 antibody [11H9]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (ischemia/reperfusion injury)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate pH6.0
      Permeabilization
      No
      Specification
      ischemia/reperfusion injury
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 14 2016

      Western blot abreview for Anti-C3 antibody [11H9]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Serum (Mouse Serum)
      Gel Running Conditions
      Reduced Denaturing (12%)
      Loading amount
      10 µg
      Specification
      Mouse Serum
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Haoran Zha

      Verified customer

      Submitted Jan 21 2016

      Question

      Has this antibody been tested for immunohistochemistry in mice using samples fixed with a Zinc fixative (non formalin) and paraffin embedded?

      Read More

      Abcam community

      Verified customer

      Asked on Jul 02 2013

      Answer

      We do not have any IHC data, either in-house or from references, for samples fixed with zinc salts fixatives, and have not received any feedback from customers who have used fixatives other than PFA and formalin. We have no reason to think that this C3 antibody will not stain samples fixed with zinc salts, or mixtures of zinc salts and formaldehyde.

      Read More

      Tom Ruyle

      Abcam Scientific Support

      Answered on Jul 02 2013

      Question

      Has this antibody ever been tested for IHC-P to detect immune deposits in human skin biopsies for the diagnosis of autoimmune diseases like lupus erythematosus?

      Read More

      Abcam community

      Verified customer

      Asked on Jul 11 2012

      Answer

      Thank you for contacting us.

      The antibody has been published in several studies that used it for detection of C3 in mouse samples but not, to our knowledge, in any studies of autoimmune disease or for detection of C3 in human samples.

      Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Jul 11 2012

      Question

      Product code: 19187
      Inquiry: Dear Support-Team, I would need to immunostain mouse/rat C3 on mouse/rat paraffin sections and thus looking for an appropriate antibody in IHC-P. Has ab19187 been tested for IHC-P as well? Given that it was raised by immunizing with purified mouse C3, it should be able to detect mouse C3. Thank you for your information. Kind regards,

      Read More

      Abcam community

      Verified customer

      Asked on May 04 2012

      Answer

      Thank you for your enquiry and your interest in our products.
      I have checked this product (ab19187: Anti-C3 antibody) for you and according to the on-line datasheet it has only been tested in Immunoelectrophoresis.
      However, we have an alternative antibody in our catalogue ab11862 which recognizes murine C3 and has been characterized for IHC-P (immunohistochemistry on formalin-fixed paraffin-embedded tissue sections).
      If you need any further assistance, please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on May 04 2012

      Question

      Dear technical team,

      Our customer is looking for C3 antibody which can detect onlybeta chain.
      I'm not sure about alpha or beta subunit so, please recommend antibody for our customer.
      His sample is human and mouse and is going to test WB with C3 antibody.
      Please infrom me.

      Best regard,

      Read More

      Abcam community

      Verified customer

      Asked on Mar 29 2012

      Answer

      Thank you for your enquiry.

      I am sorry to confirm that the C3 antibodies that detect the C3beta subunit have not been tested in WB, human and mouse.

      We do have ab11862 Anti-C3 antibody [11H9] which has been tested in WB and human and detects C3, C3b, iC3b and C3b. It should not detect C3 alpha subunit on its own.

      I have checked the alignment of the immunogen with the mouse protein, and this is 77%. We recommend that alignment should be over 85% to predict that an antibody will detect in a different species. 77% is fairly high and I would suggest there is still a possibility of some crossreactivity with mouse. However, I am sorry we are not able to guarantee this without testing.

      If the customer would like to try the antibody in mouse as part of our testing discount program, please do not hesitate to let me know and I will be pleased to arrange a discount code for them.

      I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on Mar 29 2012

      Question

      I would like to test these antibodies in rabbit in IHC-P, which has not yet been tested.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 17 2012

      Answer

      I am very pleased to hear you would like to accept our offer and test ab6678 in rabbit, ab8878 in IHC-P, and ab11862 in rabbit. These codes will give you 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for each antibody and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The codes will be active once the Abreviews have been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Jan 17 2012

      Question

      What are the molecular weights of the proteins this antibody cross-reacts with?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 13 2006

      Answer

      Thank you for your enquiry. The mature protein C3 has a molecular weight of approximately 190 kDa. The complement factor C3 consists of an alpha- and a beta-chain, linked by disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylotoxin and generating C3b (alpha chain and beta chain). C3b has a molecular weight of approximately 185 kDa. C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. iC3b has a molecular weight of approximately 182 kDa. We hope this information will be helpful to you. If you have further questions please do not hesitate to contact us.

      Read More

      Abcam Scientific Support

      Answered on Jun 15 2006

      Question

      This antibody has not worked in a western blot. The blot was tested as good using a polclonal antibody against the same protein. The serum was prepared as follows: blood was drawn by cardiac puncture (mice under anesthesia) and then placed in the"capillary serum separator" (monoject cat # 008018), let stand at RT for 45 min and then spin at 1200 g. the sup was aliquoted and stored at -80. For the western I used serum and serum that was activated; the activated serum contains cleaved C3 and a band at 46 and 35 is expected if you have a polyclonal antibody (which I saw with the polyclonal). The lot number from your ab is 152789. Have you had other complaints about this lot number/ antibody in mouse serum? Your website states that it reacts with mouse protein, what is the positive control sample that was used to test this, serum prepared as above? Please provide any additional information about how this antibody was tested.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 12 2006

      Answer

      Thank you for your patience and your e-mail regarding ab11862 monoclonal antibody to mouse C3. As a positive control affinity-purified mouse C3 was used. For the purification of mouse C3 purified antibodies were coupled to CNBr-activated Sepharose 4B. EDTA-plasma from heparinized C57BL/6 mice was applied to the column in the presence of protease inhibitor(PMSF, 10 uM). Bound complement components were eluted with 50 mM diethylamine (pH 11.5) and immediately neutralized with 1/10 volume of 500 mM NaH2PO4. Contamination by rat mAbs were removed from the eluates by protein G sepharose 4 Fast Flow in a batch procedure. For more information please see: 1) Kremmer, E et al; Monoclonal antibodies to complement complement component without the need of their prior purification. II Antibodies to mouse C3 and C4. Hybridoma 1990, 9: 309. Please note we did not receive any other complaints regarding this lot, however we take this complaint very seriously. Please contact us again if you require further assistance and do not have success with the antibody.

      Read More

      Abcam Scientific Support

      Answered on Apr 19 2006

      1-10 of 12 Abreviews or Q&A

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