Overview

  • Product name
    Anti-C3 antibody [11H9]
    See all C3 primary antibodies
  • Description
    Rat monoclonal [11H9] to C3
  • Host species
    Rat
  • Specificity
    This antibody recognizes both intact C3 and its cleaved products C3b, iC3b, C3d and C3dg. The mature protein C3 has a molecular weight of approximately 190 kDa. The complement factor C3 consists of an alpha- and a beta-chain, linked by disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylotoxin and generating C3b (alpha chain and beta chain). C3b has a molecular weight of approximately 185 kDa. C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. iC3b has a molecular weight of approximately 182 kDa. Does not cross react with C4.
  • Tested applications
    Suitable for: ELISA, ICC/IF, WB, Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1).

  • General notes

    In response to recent customer complaints for IHC-P with paraffin embedded sections we no longer guarantee this application.

Properties

Applications

Our Abpromise guarantee covers the use of ab11862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.5 µg/ml.

Use as coating antibody at 0.5 ug/well in PBS.

ICC/IF Use at an assay dependent concentration.
WB 1/50. Predicted molecular weight: 187 kDa.
Flow Cyt 1/50.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/50.

Fix tissue sections in acetone.

IP 1/50.

Target

  • Function
    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities
    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acylation stimulating protein cleavage product antibody
    • AHUS5 antibody
    • ARMD9 antibody
    • ASP antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
    • C3 antibody
    • CO3_HUMAN antibody
    • Complement C3 antibody
    • Complement C3c alpha'' chain fragment 2 antibody
    • Complement component 3 antibody
    • Complement factor 3 antibody
    • CPAMD1 antibody
    • HEL S 62p antibody
    see all

Images

  • Immunohistochemical analysis of mouse sciatic nerve section, labelling C3 with ab11862. Mice were intracardially perfused with 4% paraformaldehyde/PBS solution. Following dissection, the sciatic nerve section was embedded and cro-sectioned and immunostained with ab11862 at 1/100. 

  • ab11862 staining C3 in murine kidney cells by Immunocytochemistry/ Immunofluorescence.
    C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Glomerular staining pattern.

References

This product has been referenced in:
  • Zhao M  et al. IL-6/STAT3 pathway induced deficiency of RFX1 contributes to Th17-dependent autoimmune diseases via epigenetic regulation. Nat Commun 9:583 (2018). IHC . Read more (PubMed: 29422534) »
  • Huang B  et al. Non-erythropoietic erythropoietin-derived peptide protects mice from systemic lupus erythematosus. J Cell Mol Med 22:3330-3339 (2018). Read more (PubMed: 29570934) »
See all 16 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (ischemia/reperfusion injury)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6.0
Permeabilization
No
Specification
ischemia/reperfusion injury
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 14 2016

Application
Western blot
Sample
Mouse Serum (Mouse Serum)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
Mouse Serum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Haoran Zha

Verified customer

Submitted Jan 21 2016

Answer

We do not have any IHC data, either in-house or from references, for samples fixed with zinc salts fixatives, and have not received any feedback from customers who have used fixatives other than PFA and formalin. We have no reason to think that this C3 antibody will not stain samples fixed with zinc salts, or mixtures of zinc salts and formaldehyde.

Read More

Answer

Thank you for contacting us.

The antibody has been published in several studies that used it for detection of C3 in mouse samples but not, to our knowledge, in any studies of autoimmune disease or for detection of C3 in human samples.

Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry and your interest in our products.
I have checked this product (ab19187: Anti-C3 antibody) for you and according to the on-line datasheet it has only been tested in Immunoelectrophoresis.
However, we have an alternative antibody in our catalogue ab11862 which recognizes murine C3 and has been characterized for IHC-P (immunohistochemistry on formalin-fixed paraffin-embedded tissue sections).
If you need any further assistance, please do not hesitate to contact me.

Read More

Answer

Thank you for your enquiry.

I am sorry to confirm that the C3 antibodies that detect the C3beta subunit have not been tested in WB, human and mouse.

We do have ab11862 Anti-C3 antibody [11H9] which has been tested in WB and human and detects C3, C3b, iC3b and C3b. It should not detect C3 alpha subunit on its own.

I have checked the alignment of the immunogen with the mouse protein, and this is 77%. We recommend that alignment should be over 85% to predict that an antibody will detect in a different species. 77% is fairly high and I would suggest there is still a possibility of some crossreactivity with mouse. However, I am sorry we are not able to guarantee this without testing.

If the customer would like to try the antibody in mouse as part of our testing discount program, please do not hesitate to let me know and I will be pleased to arrange a discount code for them.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

Read More

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab6678 in rabbit, ab8878 in IHC-P, and ab11862 in rabbit. These codes will give you 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for each antibody and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The codes will be active once the Abreviews have been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question
Answer

Thank you for your enquiry. The mature protein C3 has a molecular weight of approximately 190 kDa. The complement factor C3 consists of an alpha- and a beta-chain, linked by disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylotoxin and generating C3b (alpha chain and beta chain). C3b has a molecular weight of approximately 185 kDa. C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. iC3b has a molecular weight of approximately 182 kDa. We hope this information will be helpful to you. If you have further questions please do not hesitate to contact us.

Read More

Answer

Thank you for your patience and your e-mail regarding ab11862 monoclonal antibody to mouse C3. As a positive control affinity-purified mouse C3 was used. For the purification of mouse C3 purified antibodies were coupled to CNBr-activated Sepharose 4B. EDTA-plasma from heparinized C57BL/6 mice was applied to the column in the presence of protease inhibitor(PMSF, 10 uM). Bound complement components were eluted with 50 mM diethylamine (pH 11.5) and immediately neutralized with 1/10 volume of 500 mM NaH2PO4. Contamination by rat mAbs were removed from the eluates by protein G sepharose 4 Fast Flow in a batch procedure. For more information please see: 1) Kremmer, E et al; Monoclonal antibodies to complement complement component without the need of their prior purification. II Antibodies to mouse C3 and C4. Hybridoma 1990, 9: 309. Please note we did not receive any other complaints regarding this lot, however we take this complaint very seriously. Please contact us again if you require further assistance and do not have success with the antibody.

Read More

Question
Answer

Thank you for your enquiry. I hope that all is well?The monoclonal antibody 11H9 reacts with C3, C3b, iC3b and C3d. Since C3d is a fragment of the alpha chain we presume the antibody reacts with the alpha chain of C3; however it has not been specifically verified. Have a good week end.

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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