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C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1).
In response to recent customer complaints for IHC-P with paraffin embedded sections we no longer guarantee this application.
Our Abpromise guarantee covers the use of ab11862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.5 µg/ml.
Use as coating antibody at 0.5 ug/well in PBS.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/50. Predicted molecular weight: 187 kDa.|
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Fix tissue sections in acetone.
Immunohistochemical analysis of mouse sciatic nerve section, labelling C3 with ab11862. Mice were intracardially perfused with 4% paraformaldehyde/PBS solution. Following dissection, the sciatic nerve section was embedded and cro-sectioned and immunostained with ab11862 at 1/100.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"