Key features and details
- Mouse monoclonal [8G4] to C3
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-C3 antibody [8G4]
See all C3 primary antibodies
DescriptionMouse monoclonal [8G4] to C3
SpecificityAb17455 is specific for an allotypic marker on human C3 (Leu instead of Pro in codon 314 of exon 9), which occurs regularly in the beta-chain of C3F and occasionally in the beta-chain of C3S. F and S mean fast and slow, respectively, on agarose gel electrophoresis. Ab17455 reacts with the 75-kDa beta-chain band on SDS-PAGE immunoblotting of reduced C3, and reacts with 20-kDa and 17-kDa beta-chain fragments produced by cyanogen bromide cleavage.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Full length native C3 protein isolated from human plasma.
EpitopeThe exact epitope is not known, but the antibody is specific for the allotypic human C3 where Leu is found instead of Pro in codon 314 of exon 9.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 0.0268% PBS, 2.9% Sodium chloride
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab17455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/15000. Strong reaction is seen in ELISA with C3 coated directly onto the microtiter well.|
|WB||Use at an assay dependent dilution. Detects a band of approximately 75 kDa (predicted molecular weight: 187 kDa). In Western blotting after SDS-PAGE, ab17455 detects the above-mentioned allotypic marker on the beta-chain of C3, closely related but not completely specific to the fast-moving form of C3, C3F.|
FunctionC3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
Involvement in diseaseDefects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
Sequence similaritiesContains 1 anaphylatoxin-like domain.
Contains 1 NTR domain.
modificationsC3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
Phosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- Acylation stimulating protein cleavage product antibody
- AHUS5 antibody
- ARMD9 antibody
ab17455 has been referenced in 1 publication.
- Behrendt N et al. Localization and functional significance of a polymorphic determinant in the third component of human complement. Mol Immunol 24:1097-103 (1987). PubMed: 2446123