Key features and details
- Rabbit polyclonal to C3
- Suitable for: IHC-FoFr, IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-C3 antibody
See all C3 primary antibodies
DescriptionRabbit polyclonal to C3
SpecificityThis polyclonal antibody detects a band approximately 120 kDa in Western blot under reducing conditions, corresponding to the C3 alpha chain.
Tested applicationsSuitable for: IHC-FoFr, IHC-P, IHC-Fr, WBmore details
Species reactivityReacts with: Mouse, Rat
Unfortunately, this information is considered to be commercially sensitive
- IHC-P: Mouse kidney tissue. IHC-Fr: Mouse spleen and retina tissue. WB: Fibrotic mouse liver lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab11887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/100. Detects a band of approximately 120 kDa under reducing conditions.|
FunctionC3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
Involvement in diseaseDefects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
Sequence similaritiesContains 1 anaphylatoxin-like domain.
Contains 1 NTR domain.
modificationsC3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
Phosphorylation sites are present in the extracelllular medium.
- Information by UniProt
- Acylation stimulating protein cleavage product antibody
- AHUS5 antibody
- ARMD9 antibody
Immunohistochemical analysis of rat retina tissue after bright continuous white light exposure, staining C3 with ab11887.
Lane 1 : Anti-C3 antibody (ab11887) at 1/100 dilution
Lane 2 : Anti-C3 antibody (ab11887) at 1000 cells
All lanes : Fibrotic mouse liver lysate
Lysates/proteins at 20 µg per lane.
Lane 1 : Goat anti-rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Exposure time: 1 minute
Positive staining of paraffin embedded mouse kidney tissue with ab11887 at 100x dilution.
Positive staining of frozen mouse spleen tissue with ab11887 at 20x dilution.
ab11887 has been referenced in 15 publications.
- Tian R et al. Sanqi oral solution ameliorates renal damage and restores podocyte injury in experimental membranous nephropathy via suppression of NF?B. Biomed Pharmacother 115:108904 (2019). PubMed: 31060008
- Pauly D et al. Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina. Cell Rep 29:2835-2848.e4 (2019). PubMed: 31775049
- He J et al. Microglia Mediate Synaptic Material Clearance at the Early Stage of Rats With Retinitis Pigmentosa. Front Immunol 10:912 (2019). PubMed: 31105708
- Dieguez HH et al. Superior cervical gangliectomy induces non-exudative age-related macular degeneration in mice. Dis Model Mech 11:N/A (2018). PubMed: 29361515
- Wang L et al. Mesenchymal Stem Cell-Derived Exosomes Reduce A1 Astrocytes via Downregulation of Phosphorylated NF?B P65 Subunit in Spinal Cord Injury. Cell Physiol Biochem 50:1535-1559 (2018). PubMed: 30376671