Western blot abreview for Anti-C3 antibody

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (fibrotic liver)
Loading amount
20 µg
Specification
fibrotic liver
Gel Running Conditions
Reduced Denaturing (SDS boiling for 5 min)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Other product details

Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.03% TBST with 3% BSA

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~120 kDa

Mr. Hao Wu

Verified customer

Submitted Nov 14 2012

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