• Product name
  • Description
    Rabbit polyclonal to C3
  • Host species
  • Tested applications
    Suitable for: IHC-P, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1498 - 1625 of Human C3.

  • Positive control
    • WB: HepG2 and Huh7 whole cell lysate. ICC/IF: HeLa cells. IHC: H1299 xenograft tissue; mouse brain tissue. IP: HepG2 whole cell extract.



Our Abpromise guarantee covers the use of ab97462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 187 kDa.
ICC/IF 1/100 - 1/1000.


  • Function
    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Tissue specificity
  • Involvement in disease
    Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities
    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Acylation stimulating protein cleavage product antibody
    • AHUS5 antibody
    • ARMD9 antibody
    • ASP antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
    • C3 antibody
    • CO3_HUMAN antibody
    • Complement C3 antibody
    • Complement C3c alpha'' chain fragment 2 antibody
    • Complement component 3 antibody
    • Complement factor 3 antibody
    • CPAMD1 antibody
    • HEL S 62p antibody
    see all


  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling C3 with ab97462 at 1/200 dilution (green) in ICC/IF. Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes.

    Nuclei were stained using Hoechst 33342 (blue).

  • All lanes : Anti-C3 antibody (ab97462) at 1/1000 dilution

    Lane 1 : Huh7 whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 187 kDa

    5% SDS-PAGE.

  • Paraffin-embedded mouse brain tissue stained for C3 using ab97462 at 1/500 dilution in immunohistochemical analysis.

  • C3 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts using 5 μg of ab97462. Western blot was performed from the immunoprecipitate using ab97462.

    Lane 1: HepG2 whole cell extract.

    Lane 2: Control IgG instead of ab97462 in HepG2 whole cell extract.

    Lane 3: ab97462 IP in HepG2 whole cell extract.

  • Anti-C3 antibody (ab97462) at 1/1500 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 187 kDa

    7.5% SDS-PAGE.
  • ab97462 at 1/100 dilution staining C3 in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image shows cells co-stained with Hoechst 33342.
  • ab97462 at 1/100 dilution staining C3 in H1299 xenograft by Immunohistochemistry, Paraffin-embedded tissue.


This product has been referenced in:
  • Yuen J  et al. NETosing Neutrophils Activate Complement Both on Their Own NETs and Bacteria via Alternative and Non-alternative Pathways. Front Immunol 7:137 (2016). Read more (PubMed: 27148258) »
  • Shen H  et al. Increased activity of the complement system in the liver of patients with alcoholic hepatitis. Exp Mol Pathol 97:338-44 (2014). Human . Read more (PubMed: 25217811) »

See all 5 Publications for this product

Customer reviews and Q&As

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab97462.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (tonsil, intestine)
tonsil, intestine
Antigen retrieval step
Heat mediated

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Submitted Dec 20 2011


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