• Product name

    Anti-C3 antibody (Biotin)
    See all C3 primary antibodies
  • Description

    Rabbit polyclonal to C3 (Biotin)
  • Host species

  • Conjugation

  • Tested applications

    Suitable for: IP, WB, RIAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Human C3 purified from Human plasma.



Our Abpromise guarantee covers the use of ab48342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 187 kDa.
RIA Use at an assay dependent dilution.


  • Function

    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Tissue specificity

  • Involvement in disease

    Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational

    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Acylation stimulating protein cleavage product antibody
    • AHUS5 antibody
    • ARMD9 antibody
    • ASP antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
    • C3 antibody
    • c3 complement antibody
    • C3adesArg antibody
    • CO3_HUMAN antibody
    • Complement C3 alpha chain antibody
    • Complement C3 antibody
    • Complement C3b alpha' chain antibody
    • Complement C3c alpha' chain fragment 1 antibody
    • Complement C3c alpha' chain fragment 2 antibody
    • Complement C3c alpha'' chain fragment 2 antibody
    • Complement C3d fragment antibody
    • Complement C3dg fragment antibody
    • Complement C3f fragment antibody
    • Complement C3g fragment antibody
    • Complement component 3 antibody
    • Complement factor 3 antibody
    • CPAMD1 antibody
    • HEL S 62p antibody
    • omplement C3 beta chain antibody
    see all


ab48342 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


I have attached the general Abcam protocol for Sandwich ELISAs, I know you are doing a slightly modified version but I thought it would help. As ab48342 is a purified polyclonal at a concentration at approximately 1mg/ml, I would recommend using 1-2ug of antibody to coat the wells. You may find that you will need to alter it for future experiments, but that would be my recommended starting point.

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The antibody recognizes both C3a and C3b of C3. If there is anything else I can help you with, please let me know.

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Thank you for your enquiry. I have been in touch with the source of these antibodies, and they have kindly provided the following IP protocol. Please note that this may require some further optimization in your own laboratory: IP protocol is as follows: 1. Have high binding protein A beads (from Peirce) in a column with 3 ml beads bed 2. Wash column with Glycine pH 3.5 (0.1 mM) with 2 column of volume 3. Wash 1x with 1 column volume of PBS 7.4 4. Add 400 ug of antibody to the beads and incubate for 2 hours at room temp with rotating 5. Wash with PBS 2 column volumes 6. Add cell lysates or tissue extract to the column and incubate for 2 hours at room temp. 7. Wash 2 x with PBS 8. Elute the protein and antibody with Glycine pH3.5 (0.1 mM) into the 50 ml conical tube with 250 ul of 1 M tris base as neutralization agent. (collect 25 ml) 9. Concentrate the elution liquid by lyophilization or concentrator. 10. Run SDS to determine the antigen. This method is for samples with low C3 protein expression. You can also try to coat the antibody to high binding plate and do the IP directly from the plate. The concept is similar but just smaller scale. I am sorry there are no further images to provide on this occasion. I hope this will be helpful to you. SHould you have any further questions, please do not hesitate to contact us.

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