Overview

  • Product name
    Anti-C3 / C3a antibody [2898]
    See all C3 / C3a primary antibodies
  • Description
    Mouse monoclonal [2898] to C3 / C3a
  • Host species
    Mouse
  • Specificity
    This antibody reacts with the C-terminus of C3a. It reacts with intact C3 as with C3a.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids

    CARASHLGLA

    of C3/ C3a (last nine amino acids of C3a-desArg).

  • Positive control
    • Human plasma

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    0.2 µm filtered
  • Clonality
    Monoclonal
  • Clone number
    2898
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10. Predicted molecular weight: 187.2 kDa.
 

 

Target

  • Function
    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation.
    Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:8376604, PubMed:2909530, PubMed:9059512, PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750).
  • Tissue specificity
    Plasma. The acylation stimulating protein (ASP) is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods.
  • Involvement in disease
    Complement component 3 deficiency
    Macular degeneration, age-related, 9
    Hemolytic uremic syndrome atypical 5
    Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
  • Sequence similarities
    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by insulin and dietary chylomicrons.
    Phosphorylated by FAM20C in the extracellular medium.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acylation stimulating protein antibody
    • AHUS5 antibody
    • ARMD9 antibody
    • ASP antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
    • C3 antibody
    • c3 complement antibody
    • C3adesArg antibody
    • C3bc antibody
    • CO3_HUMAN antibody
    • Complement C3 alpha chain antibody
    • Complement C3 antibody
    • Complement C3c alpha'' chain fragment 2 antibody
    • Complement component 3 antibody
    • Complement component C3 antibody
    • Complement factor 3 antibody
    • CPAMD1 antibody
    • Plp antibody
    see all

Images

  • All lanes : Anti-C3 / C3a antibody [2898] (ab11874) at 1/200 dilution

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 187.2 kDa
    Observed band size: 187 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 115 kDa, 71 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Ecker SM  et al. Aqueous cytokine and growth factor levels do not reliably reflect those levels found in the vitreous. Mol Vis 17:2856-63 (2011). WB ; Human . Read more (PubMed: 22128233) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry and I'm sorry to hear that your customer is experiencing difficulty with this antibody. Without knowing more details about what your customer has already tried, I would suggest trying the primary at 1:500 and optimizing from there based on results. To increase the signal, make sure to load at least 20-30 ug of lysate and incubate with the primary overnight at 4C. Is the customer using an Abcam secondary antibody? If the customer is still having trouble with the antibody, please send us their detailed protocol (go to the online datasheet and use the complaint link in the box under application notes).

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Answer

The antibodies our source has in their product range gave only one option for setting up an immunoassay to quantify complement C3a, but this option will also recognize C3. The option is: ab11874: monoclonal antibody to human C3/C3a (C-terminus, clone 2898) in combination with ab11872: monoclonal antibody to human C/C3a (clone 474). We know that ab11874 and ab11872 are useful as coating antibody. The usefulness of these antibodies as tracer antibody is only observed with C3 protein used as coating. However we expect that both antibodies are also useful as tracer antibody. If you are interested in these antibodies we are able to provide you with special antibodies without albumin (to use as coating and for labeling). In return we hope you are able to provide us with the test results.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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