Overview

  • Product name

    Anti-C3 / C3b antibody [2B10B9B2]
    See all C3 / C3b primary antibodies
  • Description

    Mouse monoclonal [2B10B9B2] to C3 / C3b
  • Host species

    Mouse
  • Specificity

    ab106108 reacts with an epitope on C3b. It reacts both with intact C3 and C3b.
  • Tested applications

    Suitable for: WB, ELISAmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Rat C3/ C3b

Properties

Applications

Our Abpromise guarantee covers the use of ab106108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 187 kDa.
ELISA 1/50.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
      Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • Tissue specificity

      Plasma.
    • Involvement in disease

      Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
      Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
      Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
    • Sequence similarities

      Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • Post-translational
      modifications

      C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
      Phosphorylation sites are present in the extracelllular medium.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Acylation-stimulating protein cleavage product antibody
      • AHUS5 antibody
      • ARMD9 antibody
      • ASP antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
      • C3 antibody
      • CO3_HUMAN antibody
      • Complement C3 antibody
      • Complement C3 beta chain antibody
      • Complement C3c alpha' chain fragment 2 antibody
      • Complement component 3 antibody
      • Complement component C3 antibody
      • Complement factor 3 antibody
      • CPAMD1 antibody
      • Plp antibody
      see all

    References

    ab106108 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    There are following antibodies that are best match as per your request. Please check the datasheets.

    ab11871, ab106108

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question
    Answer

    Thank you very much for your interest in ab106108.
    To our knowledge, ab106108 has not been tested in IF. Therefore, I can offer a discount off a future purchase if you buy ab106108 now, test it in IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.
    If you are interested in this offer, please follow these steps:
    1. Reply to this e-mail to let me know that you would like to proceed and test ab106108 in IF. I will then send a discount code. This code must be issued before purchasing ab106108 so please wait for my reply before ordering.
    2. Purchase ab106108 either by phone, fax, or online (www.abcam.com).
    3. Test it in IF.
    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab106108 turns out to be unsuitable for IF, you will still receive the discount on your next purchase after your Abreview has been submitted.
    Please let me know if you have any questions about this offer and I would be happy to help you further.
    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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