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    c3a-r-antibody-ab126250.pdf

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Immunology Innate Immunity Complement Classical Pathway
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Anti-C3a R antibody (ab126250)

  • Datasheet
  • SDS
Submit a review Submit a question References (2)

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Western blot - Anti-C3a R antibody (ab126250)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody (ab126250)

Key features and details

  • Rabbit polyclonal to C3a R
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human C3a R protein (ab152249)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-C3a R antibody
    See all C3a R primary antibodies
  • Description

    Rabbit polyclonal to C3a R
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human C3a R aa 1-209.

  • Positive control

    • A549 whole cell lysate
  • General notes

    Previously labelled as C3a Receptor.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Immunology
    • Innate Immunity
    • Complement
    • Alternative Pathway

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human C3a R protein (ab152249)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Chimpanzee
Non human primates
Macaque monkey
Orangutan
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. Optimal working dilutions should be determined experimentally by the user
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. Optimal working dilutions should be determined experimentally by the user
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
  • Tissue specificity

    Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q16581 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 719 Human
    • Entrez Gene: 100171704 Orangutan
    • Omim: 605246 Human
    • SwissProt: Q16581 Human
    • SwissProt: Q5REI5 Orangutan
    • Unigene: 591148 Human
    • Alternative names

      • AZ 3B antibody
      • AZ3B antibody
      • C3a anaphylatoxin chemotactic receptor antibody
      • C3A R antibody
      • C3A R1 antibody
      • C3a Receptor antibody
      • C3a-R antibody
      • C3AR antibody
      • C3AR_HUMAN antibody
      • C3AR1 antibody
      • C3R1 antibody
      • Complement component 3 receptor 1 antibody
      • Complement component 3a receptor 1 antibody
      • HNFAG09 antibody
      see all

    Images

    • Western blot - Anti-C3a R antibody (ab126250)
      Western blot - Anti-C3a R antibody (ab126250)
      Anti-C3a R antibody (ab126250) at 1/3000 dilution + A549 whole cell lysate at 30 µg

      Predicted band size: 54 kDa



      10% SDS-PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody (ab126250)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody (ab126250)
      ab126250, at 1/500 dilution, staining paraffin-embedded colon ca by immunohistochemical analysis

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab126250? Please let us know so that we can cite the reference in this datasheet.

    ab126250 has been referenced in 2 publications.

    • Huang X  et al. Neuronal complement cascade drives bone cancer pain via C3R mediated microglial activation. Brain Res 1698:81-88 (2018). PubMed: 29909203
    • Wang X  et al. Evaluation of expression levels and mechanism of complement activation. Exp Ther Med 14:2493-2496 (2017). PubMed: 28962185

    Customer reviews and Q&As

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