Anti-C3a R antibody (ab126250)
Key features and details
- Rabbit polyclonal to C3a R
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C3a R antibody
See all C3a R primary antibodies -
Description
Rabbit polyclonal to C3a R -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment corresponding to Human C3a R aa 1-209.
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Positive control
- A549 whole cell lysate
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General notes
Previously labelled as C3a Receptor.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Chimpanzee
Non human primates
Macaque monkey
Orangutan
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 54 kDa. Optimal working dilutions should be determined experimentally by the user
|
|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. Optimal working dilutions should be determined experimentally by the user |
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. -
Tissue specificity
Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsAmong the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 719 Human
- Entrez Gene: 100171704 Orangutan
- Omim: 605246 Human
- SwissProt: Q16581 Human
- SwissProt: Q5REI5 Orangutan
- Unigene: 591148 Human
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Alternative names
- AZ 3B antibody
- AZ3B antibody
- C3a anaphylatoxin chemotactic receptor antibody
see all
Images
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Western blot - Anti-C3a R antibody (ab126250)Anti-C3a R antibody (ab126250) at 1/3000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3a R antibody (ab126250)ab126250, at 1/500 dilution, staining paraffin-embedded colon ca by immunohistochemical analysis
Protocols
References (2)
ab126250 has been referenced in 2 publications.
- Huang X et al. Neuronal complement cascade drives bone cancer pain via C3R mediated microglial activation. Brain Res 1698:81-88 (2018). PubMed: 29909203
- Wang X et al. Evaluation of expression levels and mechanism of complement activation. Exp Ther Med 14:2493-2496 (2017). PubMed: 28962185
