Key features and details
- Mouse monoclonal [7C12] to C3b / iC3b
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-C3b / iC3b antibody [7C12]
See all C3b / iC3b primary antibodies
DescriptionMouse monoclonal [7C12] to C3b / iC3b
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human C3b/ iC3b. C3b(i)-Sepharose. C3bi was deposited on Sepharose 4B via the alternative pathway of complement activation in normal human serum.
- WB: human serum and plasma lysates, human liver whole tissue lysate
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact email@example.com
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab231078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.|
FunctionC3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation.
Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed:8376604, PubMed:2909530, PubMed:9059512, PubMed:10432298, PubMed:15833747, PubMed:16333141, PubMed:19615750).
Tissue specificityPlasma. The acylation stimulating protein (ASP) is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods.
Involvement in diseaseComplement component 3 deficiency
Macular degeneration, age-related, 9
Hemolytic uremic syndrome atypical 5
Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
Sequence similaritiesContains 1 anaphylatoxin-like domain.
Contains 1 NTR domain.
modificationsC3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by insulin and dietary chylomicrons.
Phosphorylated by FAM20C in the extracellular medium.
- Information by UniProt
- ASP antibody
- C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
- C3 antibody
All lanes : Anti-C3b / iC3b antibody [7C12] (ab231078) at 1 µg/ml
Lane 1 : Human Sera diluted 1/1000 at 10 µl
Lane 2 : Purified C3 Protein at 0.1 µg
Lane 3 : Purified C3b Protein at 0.1 µg
Lane 4 : Purified iC3b Protein at 0.1 µg
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 110,125,43,78 kDa why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk before being incubated with ab231078 overnight at 4°C at 1ug/ml. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes :
Lane 1 : Human serum diluted 1/100 at 5 µl
Lane 2 : Human plasma diluted 1/100 at 5 µl
Lane 3 : Human liver whole tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 68 kDa
Additional bands at: 42 kDa (possible cleavage fragment)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk before ab231078 and ab181602 (Rabbit anti GAPDH) were incubated overnight at 4°C at a 1ug/ml concentration and 1/10000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231078 has been referenced in 4 publications.
- Lindorfer MA et al. A novel approach to preventing the hemolysis of paroxysmal nocturnal hemoglobinuria: both complement-mediated cytolysis and C3 deposition are blocked by a monoclonal antibody specific for the alternative pathway of complement. Blood 115:2283-91 (2010). PubMed: 20068220
- Kennedy AD et al. Rituximab infusion promotes rapid complement depletion and acute CD20 loss in chronic lymphocytic leukemia. J Immunol 172:3280-8 (2004). PubMed: 14978136
- Kennedy AD et al. An anti-C3b(i) mAb enhances complement activation, C3b(i) deposition, and killing of CD20+ cells by rituximab. Blood 101:1071-9 (2003). PubMed: 12393727
- Tosic L et al. Preparation of monoclonal antibodies to C3b by immunization with C3b(i)-sepharose. J Immunol Methods 120:241-9 (1989). PubMed: 2786912