• Product name

    Anti-C3d antibody [7C10]
    See all C3d primary antibodies
  • Description

    Mouse monoclonal [7C10] to C3d
  • Host species

  • Specificity

    This product is specific to C3d, but also C3b and iC3b, since C3d is a product from C3b.

  • Tested applications

    Suitable for: IHC, ELISA, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human C3d.

  • Epitope

    Epitope specificity differs from that of ab17455.
  • Positive control

    • IHC-P: Human kidney tissue.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.098% Sodium azide
    Constituents: PBS, 2.9% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

  • Clone number

  • Myeloma

  • Isotype

  • Light chain type

  • Research areas


Our Abpromise guarantee covers the use of ab17453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IF 1/10.
IHC 1/10.
ELISA 1/30000. Strong reaction is seen in ELISA with a C3 coat or when used as detection antibody in sandwich ELISA in combination with a polyclonal C3 antibody. ab17453 also reacts with C3b deposited on coated antibody molecules.
WB Use at an assay dependent concentration. Predicted molecular weight: 187 kDa. In Western blotting after SDS-PAGE, ab17453 reacts with C3 in both reduced and unreduced forms.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function

    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Tissue specificity

  • Involvement in disease

    Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational

    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Acylation stimulating protein cleavage product antibody
    • AHUS5 antibody
    • ARMD9 antibody
    • ASP antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
    • C3 antibody
    • c3 complement antibody
    • CO3_HUMAN antibody
    • Complement C3 antibody
    • Complement C3c alpha' chain fragment 2 antibody
    • Complement C3d fragment antibody
    • Complement component 3 antibody
    • Complement component C3 antibody
    • CPAMD1 antibody
    see all


  • Immunohistochemical analysis of human kidney labelling C3d with ab17453 at a dilution of 1/10. Plasma of veins were stained strongly.

  • Immunofluorescent analysis of human kidney labelling C3d with ab17453 at a dilution of 1/10. Plasma of veins were stained strongly.


This product has been referenced in:

  • Pathak A  et al. Factor H binding proteins protect division septa on encapsulated Streptococcus pneumoniae against complement C3b deposition and amplification. Nat Commun 9:3398 (2018). Read more (PubMed: 30139996) »
  • Hu C  et al. Complement Inhibitor CRIg/FH Ameliorates Renal Ischemia Reperfusion Injury via Activation of PI3K/AKT Signaling. J Immunol 201:3717-3730 (2018). Read more (PubMed: 30429287) »
See all 6 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Diseased mouse kidney | paraffin sections)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Diseased mouse kidney | paraffin sections
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 22°C

Mr. Theodore Kaplan

Verified customer

Submitted Dec 21 2016


While the optimal concentration for use of this product will need to be determined experimentally in the lab, we recommend a starting dilution of 1ug/ml in Western blot.

Read More


Unfortunately I cannot not find much info on the epitope of this antibody. What I can say is that is will probable recognize C3d no matter how it is formed (could be LPS activation). As I do not know where the epitope is, I do not know if it will recognize C3dg, but I think not.

Read More
Flow Cytometry
Human Cell (ovary)
Cell harvesting/tissue preparation method: trypsinization
Sample buffer: PBS
Gating Strategy
live cells

Abcam user community

Verified customer

Submitted Dec 11 2007

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