Key features and details
- Rabbit polyclonal to C3orf25
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C3orf25 antibody
DescriptionRabbit polyclonal to C3orf25
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- RT4, U-251 MG and Human Liver lysates, Human pancreas tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
- C3orf25 antibody
- EF-hand domain-containing protein C3orf25 antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus. Recommended concentration of ab122408 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-C3orf25 antibody (ab122408) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
10-20% SDS PAGE
ab122408 at 1/200 dilution staining C3orf25 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
ab122408 has not yet been referenced specifically in any publications.