Product nameAnti-C3orf37 antibody
DescriptionRabbit polyclonal to C3orf37
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human testis tissue. IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Sequence similaritiesBelongs to the UPF0361 family.
- Information by UniProt
- 5-Hydroxymethylcytosine (HmC) Binding, ES Cell-Specific antibody
- C3orf37 antibody
- CC037_HUMAN antibody
Paraffin-embedded formalin-fixed human testis tissue stained for C3orf37 using ab272646 at 1/50 dilution in immunohistochemical analysis.
PFA fixed, Triton X-100 permeablized U-2 OS cells (human bone osteosarcoma epithelial cell line) labeling C3orf37 (green) using ab272646 at 2 ug/ml in ICC/IF analysis.
All lanes : Anti-C3orf37 antibody (ab272646) at 0.4 µg/ml
Lane 1 : Control siRNA transfected into MCF-7 cells (Human breast adenocarcinoma cell line)
Lane 2 : siRNA probe #1 transfected into MCF-7 cells
Lane 3 : siRNA probe #2 transfected into MCF-7 cells
Loading control: Anti-GAPDH.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272646 has not yet been referenced specifically in any publications.