Overview

  • Product name
    Anti-C4 antibody [16D2]
    See all C4 primary antibodies
  • Description
    Rat monoclonal [16D2] to C4
  • Host species
    Rat
  • Specificity
    Antibody cross reacts with C4, C4b and C4d.
  • Tested applications
    Suitable for: WB, IHC-Fr, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    This antibody reacts with intact C4, C4b and C4d.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    16D2
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10. Predicted molecular weight: 192 kDa.
IHC-Fr 1/10.
IP 1/10.
Flow Cyt 1/10.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Information by UniProt
  • Database links
  • Alternative names
    • Acidic C4 antibody
    • Acidic complement C4 antibody
    • Basic C4 antibody
    • Basic complement C4 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
    • C4, Chido form antibody
    • C4, Rodgers from antibody
    • C4A anaphylatoxin antibody
    • C4A antibody
    • C4A2 antibody
    • C4A3 antibody
    • C4A4 antibody
    • C4A6 antibody
    • C4AD antibody
    • C4B antibody
    • C4B_2 antibody
    • C4B1 antibody
    • C4B12 antibody
    • C4B2 antibody
    • C4B3 antibody
    • C4BD antibody
    • C4F antibody
    • C4S antibody
    • CH antibody
    • Chido form of C4 antibody
    • CO4 antibody
    • CO4A_HUMAN antibody
    • Complement C4 gamma chain antibody
    • complement C4-A antibody
    • complement C4-B antibody
    • complement C4-B-like antibody
    • complement C4B1a antibody
    • Complement component 4A (Rodgers blood group) antibody
    • Complement component 4A antibody
    • complement component 4B (Chido blood group), copy 2 antibody
    • Complement component 4B (Childo blood group) antibody
    • Complement component 4B antibody
    • Complement component 4F antibody
    • Complement component 4S antibody
    • CPAMD2 antibody
    • CPAMD3 antibody
    • RG antibody
    • Rodgers form of C4 antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) analysis of mouse kidney allografts (left) and normal kidney tissue labelling C4 with ab11863 at 1/50. Staining seen predominantly in peritubular capillary walls. Magnification: X250.

    4μm frozen sections were fixed in acetone and blocked in normal goat serum. A FITC-conjugated goat anti-rat IgG was used as the secondary antibody.

References

This product has been referenced in:
  • Garland DL  et al. Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration. Hum Mol Genet N/A:N/A (2013). Mouse . Read more (PubMed: 23943789) »
  • O'Connor T  et al. Lymphotoxin, but not TNF, is required for prion invasion of lymph nodes. PLoS Pathog 8:e1002867 (2012). Read more (PubMed: 22912582) »
See all 6 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Assuming you have not already tried this clone, 16D2, the antibody ab11863 should be capable of detecting mouse C4, but we do not know how much is expected to be present in brain tissue. We recommend a positive control of, for instance, mouse kidney. The following reference includes IHC data and a protocol for mouse kidney tissue that should be applicable to brain tissue. Please note though that the tissue that was stained for C4 was not PFA-perfused or formalin-fixed, just frozen and fixed on the slides with acetone.

Am J Pathol. Apr 2006; 168(4): 1241–1248.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1606553/

There are two other references listed on the datasheet that may be useful for mouse IHC. Here is a link:

https://www.abcam.com/c4-antibody-16d2-ab11863-references.html

Read More
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (kidney)
Specification
kidney
Treatment
allografted
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 19 2014

Answer

Thank you for contacting us. I have been doing a little research into C4 and the antibodies that we currently have available against this target. As discussed on the phone, ab89537 recognises mouse C4d and C4 and has been tested in flow cytometry and ICC. We also have a rat monoclonal (ab11863), which is directed against mouse C4, and recognises C4b and C4d and has been used with flow cytometry and IHC-Fr. Unfortunately as human C4d only shares 82% homology with mouse C4d, the antibodies directed against human C4d are unlikely to react. From my understanding, C4 is cleaved into C4a and C4b by C1. C4b is then cleaved further to form C4c and C4d. An antibody which only recognises C4d (and not C4) is therefore unlikely to happen as the amino acid sequence of C4d is included in that of C4. Or did I misunderstand the question and were you asking for specificity against another isoform? I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. The following reference has used the antibody and gives details of its characterization: Murata, K et al; Synergistic deposition of C4d by complement-activating and non-activating antibodies in cardiac transplants. Am J Transplant 2007, 7: 2605 It may be necessary to activate the complement cascade to observe C4. It would suggest searching the literature for further information. We do have the human protein, but this antibody is directed against mouse so may not cross-react (ab98983-C4 protein). If you have further questions, please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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