Overview

  • Product name
    Anti-C4 antibody [16D2]
    See all C4 primary antibodies
  • Description
    Rat monoclonal [16D2] to C4
  • Host species
    Rat
  • Specificity
    Antibody cross reacts with C4, C4b and C4d.
  • Tested applications
    Suitable for: WB, IHC-Fr, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    This antibody reacts with intact C4, C4b and C4d.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    16D2
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10. Predicted molecular weight: 192 kDa.
IHC-Fr 1/10.
IP 1/10.
Flow Cyt 1/10.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Information by UniProt
  • Database links
    • Alternative names
      • Acidic C4 antibody
      • Acidic complement C4 antibody
      • Basic C4 antibody
      • Basic complement C4 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
      • C4, Chido form antibody
      • C4, Rodgers from antibody
      • C4A anaphylatoxin antibody
      • C4A antibody
      • C4A2 antibody
      • C4A3 antibody
      • C4A4 antibody
      • C4A6 antibody
      • C4AD antibody
      • C4B antibody
      • C4B_2 antibody
      • C4B1 antibody
      • C4B12 antibody
      • C4B2 antibody
      • C4B3 antibody
      • C4BD antibody
      • C4F antibody
      • C4S antibody
      • CH antibody
      • Chido form of C4 antibody
      • CO4 antibody
      • CO4A_HUMAN antibody
      • Complement C4 gamma chain antibody
      • complement C4-A antibody
      • complement C4-B antibody
      • complement C4-B-like antibody
      • complement C4B1a antibody
      • Complement component 4A (Rodgers blood group) antibody
      • Complement component 4A antibody
      • complement component 4B (Chido blood group), copy 2 antibody
      • Complement component 4B (Childo blood group) antibody
      • Complement component 4B antibody
      • Complement component 4F antibody
      • Complement component 4S antibody
      • CPAMD2 antibody
      • CPAMD3 antibody
      • RG antibody
      • Rodgers form of C4 antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-C4 antibody [16D2] (ab11863)
      Immunohistochemistry (Frozen sections) - Anti-C4 antibody [16D2] (ab11863)Image from Lin T et al., Am J Pathol. 2006;168(4):1241-8. Fig 2.; doi: 10.2353/ajpath.2006.050360 with permission from Elsevier.

      Immunohistochemistry (Frozen sections) analysis of mouse kidney allografts (left) and normal kidney tissue labelling C4 with ab11863 at 1/50. Staining seen predominantly in peritubular capillary walls. Magnification: X250.

      4μm frozen sections were fixed in acetone and blocked in normal goat serum. A FITC-conjugated goat anti-rat IgG was used as the secondary antibody.

    References

    This product has been referenced in:
    • Garland DL  et al. Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration. Hum Mol Genet N/A:N/A (2013). Mouse . Read more (PubMed: 23943789) »
    • O'Connor T  et al. Lymphotoxin, but not TNF, is required for prion invasion of lymph nodes. PLoS Pathog 8:e1002867 (2012). Read more (PubMed: 22912582) »

    See all 6 Publications for this product

    Publishing research using ab11863? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    4 reviews or Q&A

    10
    Votes: Highest First

    I'm writing to ask if your C4 antibody (https://www.abcam.com/c4-antibody-16d2-ab11863.html) works in mouse brain tissue. I've been screening C4 antibodies in mouse brain but have been unable to get one to work. Do you think your antibody is worth tryi...

    Read More

    Assuming you have not already tried this clone, 16D2, the antibody ab11863 should be capable of detecting mouse C4, but we do not know how much is expected to be present in brain tissue. We recommend a positive control of, for instance, mouse kidney. T...

    Read More

    Western blot abreview for Anti-C4 antibody [16D2]

     Good
    Abreviews
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (kidney)
    Specification
    kidney
    Treatment
    allografted
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Aug 19 2014

    Customer called call regarding antibodies against C4d, in particular ab89537 and if it is specific for C4d (and not C4). Or if we have any antibodies specific for this with mouse samples. With application in flow/IHC.

    Thank you for contacting us. I have been doing a little research into C4 and the antibodies that we currently have available against this target. As discussed on the phone, ab89537 recognises mouse C4d and C4 and has been tested in flow cytomet...

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    Hi, I would like to purchase this antibody, but would like to know what tissue to use as a positive control. Thank you!

    Thank you for contacting us. The following reference has used the antibody and gives details of its characterization: Murata, K et al; Synergistic deposition of C4d by complement-activating and non-activating antibodies in cardiac transplants. ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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