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    c4-antibody-16d2-ab11863.pdf

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Immunology Innate Immunity Complement Classical Pathway
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Anti-C4 antibody [16D2] (ab11863)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (11)

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Immunohistochemistry (Frozen sections) - Anti-C4 antibody [16D2] (ab11863)

    Key features and details

    • Rat monoclonal [16D2] to C4
    • Suitable for: IHC-Fr
    • Reacts with: Mouse
    • Isotype: IgG2a

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    Anti-C3 / C3b antibody [755] (ab11871)
    Secondary
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    Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    Protein
    Recombinant Human C4 protein (ab199588)

    View more associated products

    Overview

    • Product name

      Anti-C4 antibody [16D2]
      See all C4 primary antibodies
    • Description

      Rat monoclonal [16D2] to C4
    • Host species

      Rat
    • Specificity

      Antibody cross reacts with C4, C4b and C4d.
    • Tested applications

      Suitable for: IHC-Frmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      This information is considered to be commercially sensitive.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituents: PBS, 0.1% BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      16D2
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Innate Immunity
      • Complement
      • Classical Pathway

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Isotype control

      • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
    • Recombinant Protein

      • Recombinant Human C4 protein (ab199588)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab11863 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr
    Use at an assay dependent concentration.
    Notes
    IHC-Fr
    Use at an assay dependent concentration.

    Target

    • Target information above from: UniProt accession P0C0L4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12268 Mouse
      • Entrez Gene: 625018 Mouse
      • Entrez Gene: 675521 Mouse
      • SwissProt: P01029 Mouse
      • Unigene: 439678 Mouse
      • Unigene: 477109 Mouse
      • Alternative names

        • Acidic C4 antibody
        • Acidic complement C4 antibody
        • Basic C4 antibody
        • Basic complement C4 antibody
        • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
        • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
        • C4, Chido form antibody
        • C4, Rodgers from antibody
        • C4A anaphylatoxin antibody
        • C4A antibody
        • C4A2 antibody
        • C4A3 antibody
        • C4A4 antibody
        • C4A6 antibody
        • C4AD antibody
        • C4B antibody
        • C4B_2 antibody
        • C4B1 antibody
        • C4B12 antibody
        • C4B2 antibody
        • C4B3 antibody
        • C4BD antibody
        • C4F antibody
        • C4S antibody
        • CH antibody
        • Chido form of C4 antibody
        • CO4 antibody
        • CO4A_HUMAN antibody
        • Complement C4 A antibody
        • Complement C4 B antibody
        • Complement C4 gamma chain antibody
        • complement C4-A antibody
        • complement C4-B antibody
        • complement C4-B-like antibody
        • complement C4B1a antibody
        • Complement component 4A (Rodgers blood group) antibody
        • Complement component 4A antibody
        • complement component 4B (Chido blood group) antibody
        • complement component 4B (Chido blood group), copy 2 antibody
        • Complement component 4B (Childo blood group) antibody
        • Complement component 4B antibody
        • Complement component 4F antibody
        • Complement component 4S antibody
        • CPAMD2 antibody
        • CPAMD3 antibody
        • RG antibody
        • Rodgers form of C4 antibody
        see all

      Images

      • Immunohistochemistry (Frozen sections) - Anti-C4 antibody [16D2] (ab11863)
        Immunohistochemistry (Frozen sections) - Anti-C4 antibody [16D2] (ab11863)Image from Lin T et al., Am J Pathol. 2006;168(4):1241-8. Fig 2.; doi: 10.2353/ajpath.2006.050360 with permission from Elsevier.

        Immunohistochemistry (Frozen sections) analysis of mouse kidney allografts (left) and normal kidney tissue labelling C4 with ab11863 at 1/50. Staining seen predominantly in peritubular capillary walls. Magnification: X250.

        4μm frozen sections were fixed in acetone and blocked in normal goat serum. A FITC-conjugated goat anti-rat IgG was used as the secondary antibody.

      Protocols

      • Flow cytometry protocols
      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (11)

      Publishing research using ab11863? Please let us know so that we can cite the reference in this datasheet.

      ab11863 has been referenced in 11 publications.

      • Li Y  et al. Persistent Toxoplasma Infection of the Brain Induced Neurodegeneration Associated with Activation of Complement and Microglia. Infect Immun 87:N/A (2019). PubMed: 31182619
      • Smith KL  et al. Microglial cell hyper-ramification and neuronal dendritic spine loss in the hippocampus and medial prefrontal cortex in a mouse model of PTSD. Brain Behav Immun 80:889-899 (2019). PubMed: 31158497
      • Harper IG  et al. Augmentation of Recipient Adaptive Alloimmunity by Donor Passenger Lymphocytes within the Transplant. Cell Rep 15:1214-27 (2016). PubMed: 27134179
      • Shinoda K  et al. Depletion of foxp3(+) T cells abrogates tolerance of skin and heart allografts in murine mixed chimeras without the loss of mixed chimerism. Am J Transplant 14:2263-2274 (2014). PubMed: 25155089
      • Garland DL  et al. Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration. Hum Mol Genet N/A:N/A (2013). Mouse . PubMed: 23943789
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Question

      I'm writing to ask if your C4 antibody (https://www.abcam.com/c4-antibody-16d2-ab11863.html) works in mouse brain tissue. I've been screening C4 antibodies in mouse brain but have been unable to get one to work. Do you think your antibody is worth trying for this application?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 03 2014

      Answer

      Assuming you have not already tried this clone, 16D2, the antibody ab11863 should be capable of detecting mouse C4, but we do not know how much is expected to be present in brain tissue. We recommend a positive control of, for instance, mouse kidney. The following reference includes IHC data and a protocol for mouse kidney tissue that should be applicable to brain tissue. Please note though that the tissue that was stained for C4 was not PFA-perfused or formalin-fixed, just frozen and fixed on the slides with acetone.

      Am J Pathol. Apr 2006; 168(4): 1241–1248.

      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1606553/

      There are two other references listed on the datasheet that may be useful for mouse IHC. Here is a link:

      https://www.abcam.com/c4-antibody-16d2-ab11863-references.html

      Read More

      Tom Ruyle

      Abcam Scientific Support

      Answered on Apr 03 2014

      Western blot abreview for Anti-C4 antibody [16D2]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      25 µg
      Gel Running Conditions
      Reduced Denaturing
      Sample
      Mouse Tissue lysate - whole (kidney)
      Specification
      kidney
      Treatment
      allografted
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 19 2014

      Question

      Customer called call regarding antibodies against C4d, in particular ab89537 and if it is specific for C4d (and not C4). Or if we have any antibodies specific for this with mouse samples. With application in flow/IHC.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 12 2011

      Answer

      Thank you for contacting us. I have been doing a little research into C4 and the antibodies that we currently have available against this target. As discussed on the phone, ab89537 recognises mouse C4d and C4 and has been tested in flow cytometry and ICC. We also have a rat monoclonal (ab11863), which is directed against mouse C4, and recognises C4b and C4d and has been used with flow cytometry and IHC-Fr. Unfortunately as human C4d only shares 82% homology with mouse C4d, the antibodies directed against human C4d are unlikely to react. From my understanding, C4 is cleaved into C4a and C4b by C1. C4b is then cleaved further to form C4c and C4d. An antibody which only recognises C4d (and not C4) is therefore unlikely to happen as the amino acid sequence of C4d is included in that of C4. Or did I misunderstand the question and were you asking for specificity against another isoform? I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Oct 12 2011

      Question

      Hi, I would like to purchase this antibody, but would like to know what tissue to use as a positive control. Thank you!

      Read More

      Abcam community

      Verified customer

      Asked on Mar 04 2011

      Answer

      Thank you for contacting us. The following reference has used the antibody and gives details of its characterization: Murata, K et al; Synergistic deposition of C4d by complement-activating and non-activating antibodies in cardiac transplants. Am J Transplant 2007, 7: 2605 It may be necessary to activate the complement cascade to observe C4. It would suggest searching the literature for further information. We do have the human protein, but this antibody is directed against mouse so may not cross-react (ab98983-C4 protein). If you have further questions, please do not hesitate to contact us.

      Read More

      Abcam Scientific Support

      Answered on Mar 04 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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