Anti-C4 antibody [16D2] (ab11863)
Key features and details
- Rat monoclonal [16D2] to C4
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-C4 antibody [16D2]
See all C4 primary antibodies -
Description
Rat monoclonal [16D2] to C4 -
Host species
Rat -
Specificity
Antibody cross reacts with C4, C4b and C4d. -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
This information is considered to be commercially sensitive.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
16D2 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 12268 Mouse
- Entrez Gene: 625018 Mouse
- Entrez Gene: 675521 Mouse
- SwissProt: P01029 Mouse
- Unigene: 439678 Mouse
- Unigene: 477109 Mouse
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Alternative names
- Acidic C4 antibody
- Acidic complement C4 antibody
- Basic C4 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-C4 antibody [16D2] (ab11863)Image from Lin T et al., Am J Pathol. 2006;168(4):1241-8. Fig 2.; doi: 10.2353/ajpath.2006.050360 with permission from Elsevier.
Immunohistochemistry (Frozen sections) analysis of mouse kidney allografts (left) and normal kidney tissue labelling C4 with ab11863 at 1/50. Staining seen predominantly in peritubular capillary walls. Magnification: X250.
4μm frozen sections were fixed in acetone and blocked in normal goat serum. A FITC-conjugated goat anti-rat IgG was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab11863 has been referenced in 11 publications.
- Li Y et al. Persistent Toxoplasma Infection of the Brain Induced Neurodegeneration Associated with Activation of Complement and Microglia. Infect Immun 87:N/A (2019). PubMed: 31182619
- Smith KL et al. Microglial cell hyper-ramification and neuronal dendritic spine loss in the hippocampus and medial prefrontal cortex in a mouse model of PTSD. Brain Behav Immun 80:889-899 (2019). PubMed: 31158497
- Harper IG et al. Augmentation of Recipient Adaptive Alloimmunity by Donor Passenger Lymphocytes within the Transplant. Cell Rep 15:1214-27 (2016). PubMed: 27134179
- Shinoda K et al. Depletion of foxp3(+) T cells abrogates tolerance of skin and heart allografts in murine mixed chimeras without the loss of mixed chimerism. Am J Transplant 14:2263-2274 (2014). PubMed: 25155089
- Garland DL et al. Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration. Hum Mol Genet N/A:N/A (2013). Mouse . PubMed: 23943789