Product nameAnti-C4 antibody
See all C4 primary antibodies
DescriptionChicken polyclonal to C4
Specificityab48582 bind to 3 bands (88,75 and 33 kDa) of human C4B. May cross react with forms of C4b from other mammalian species.
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Purified human complement C4B.
- This antibody gave a positive result in IHC in the following FFPE tissue: Human Liver.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab48582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000. Detects bands of approximately 33, 75 and 88 kDa (predicted molecular weight: 192 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Information by UniProt
- Acidic C4 antibody
- Acidic complement C4 antibody
- Basic C4 antibody
IHC image of C4 staining in Human Liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab48582, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab48582 has not yet been referenced specifically in any publications.