• Product name

  • Description

    Chicken polyclonal to C4
  • Host species

  • Specificity

    ab48582 bind to 3 bands (88,75 and 33 kDa) of human C4B. May cross react with forms of C4b from other mammalian species.
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Purified human complement C4B.

  • Positive control

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human Liver.


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab48582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: 1/1000 - 1/5000.
    WB: 1/1000 - 1/5000. Detects bands of approximately 33, 75 and 88 kDa (predicted molecular weight: 192 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Information by UniProt
    • Database links

    • Alternative names

      • Acidic C4 antibody
      • Acidic complement C4 antibody
      • Basic C4 antibody
      • Basic complement C4 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
      • C4, Chido form antibody
      • C4, Rodgers from antibody
      • C4A anaphylatoxin antibody
      • C4A antibody
      • C4A2 antibody
      • C4A3 antibody
      • C4A4 antibody
      • C4A6 antibody
      • C4AD antibody
      • C4B antibody
      • C4B_2 antibody
      • C4B1 antibody
      • C4B12 antibody
      • C4B2 antibody
      • C4B3 antibody
      • C4BD antibody
      • C4F antibody
      • C4S antibody
      • CH antibody
      • Chido form of C4 antibody
      • CO4 antibody
      • CO4A_HUMAN antibody
      • Complement C4 A antibody
      • Complement C4 B antibody
      • Complement C4 gamma chain antibody
      • complement C4-A antibody
      • complement C4-B antibody
      • complement C4-B-like antibody
      • complement C4B1a antibody
      • Complement component 4A (Rodgers blood group) antibody
      • Complement component 4A antibody
      • complement component 4B (Chido blood group) antibody
      • complement component 4B (Chido blood group), copy 2 antibody
      • Complement component 4B (Childo blood group) antibody
      • Complement component 4B antibody
      • Complement component 4F antibody
      • Complement component 4S antibody
      • CPAMD2 antibody
      • CPAMD3 antibody
      • RG antibody
      • Rodgers form of C4 antibody
      see all


    • IHC image of C4 staining in Human Liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab48582, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


    ab48582 has not yet been referenced specifically in any publications.

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