Product nameAnti-C4 antibody [EPR2990(2)]
See all C4 primary antibodies
DescriptionRabbit monoclonal [EPR2990(2)] to C4
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human C4 aa 1200-1300 (Cysteine residue). The exact sequence is proprietary. Note: this sequence is identical in both C4 subunits A (P0C0L4) and B (P0C0L5).
Database link: P0C0L4
- WB: HepG2 cell lysate. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab173577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 193 kDa.|
|ICC/IF||1/100 - 1/250.|
- Information by UniProt
- Acidic C4 antibody
- Acidic complement C4 antibody
- Basic C4 antibody
Anti-C4 antibody [EPR2990(2)] (ab173577) at 1/1000 dilution + HepG2 cell lysate at 10 µg
Predicted band size: 193 kDa
Immunofluorescent staining of HepG2 cells labeling C4 with ab173577 at 1/100
Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling C4 (red) with ab173577 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.