Overview

  • Product name
    Anti-C4 antibody [EPR2990(2)]
    See all C4 primary antibodies
  • Description
    Rabbit monoclonal [EPR2990(2)] to C4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human C4 aa 1200-1300 (Cysteine residue). The exact sequence is proprietary. Note: this sequence is identical in both C4 subunits A (P0C0L4) and B (P0C0L5).
    Database link: P0C0L4

  • Positive control
    • WB: HepG2 cell lysate. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR2990(2)
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab173577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 193 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/20.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Information by UniProt
    • Database links
    • Alternative names
      • Acidic C4 antibody
      • Acidic complement C4 antibody
      • Basic C4 antibody
      • Basic complement C4 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
      • C4, Chido form antibody
      • C4, Rodgers from antibody
      • C4A anaphylatoxin antibody
      • C4A antibody
      • C4A2 antibody
      • C4A3 antibody
      • C4A4 antibody
      • C4A6 antibody
      • C4AD antibody
      • C4B antibody
      • C4B_2 antibody
      • C4B1 antibody
      • C4B12 antibody
      • C4B2 antibody
      • C4B3 antibody
      • C4BD antibody
      • C4F antibody
      • C4S antibody
      • CH antibody
      • Chido form of C4 antibody
      • CO4 antibody
      • CO4A_HUMAN antibody
      • Complement C4 gamma chain antibody
      • complement C4-A antibody
      • complement C4-B antibody
      • complement C4-B-like antibody
      • complement C4B1a antibody
      • Complement component 4A (Rodgers blood group) antibody
      • Complement component 4A antibody
      • complement component 4B (Chido blood group), copy 2 antibody
      • Complement component 4B (Childo blood group) antibody
      • Complement component 4B antibody
      • Complement component 4F antibody
      • Complement component 4S antibody
      • CPAMD2 antibody
      • CPAMD3 antibody
      • RG antibody
      • Rodgers form of C4 antibody
      see all

    Images

    • Immunofluorescent staining of HepG2 cells labeling C4 with ab173577 at 1/100

    • Anti-C4 antibody [EPR2990(2)] (ab173577) at 1/1000 dilution + HepG2 cell lysate at 10 µg

      Predicted band size: 193 kDa

    • Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling C4 (red) with ab173577 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    References

    This product has been referenced in:
    • Sellgren CM  et al. Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors. Mol Psychiatry 22:170-177 (2017). WB, ICC/IF ; Human . Read more (PubMed: 27956744) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab173577.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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