Recombinant Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17101] to C4 binding protein/C4BPB
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-C4 binding protein/C4BPB antibody [EPR17101]
See all C4 binding protein/C4BPB primary antibodies -
Description
Rabbit monoclonal [EPR17101] to C4 binding protein/C4BPB -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human plasma and human ovary cancer cell extracts. Human serum. Rat plasma. IHC-P: Human hepatocellular carcinoma and human bladder carcinoma tissue. Rat liver tissue. ICC/IF: HepG2 cells. IP: Human serum.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17101 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 28 kDa).
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IP |
1/80.
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ICC/IF |
1/250.
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Notes |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 28 kDa). |
IP
1/80. |
ICC/IF
1/250. |
Target
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Function
Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S. -
Sequence similarities
Contains 3 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 725 Human
- Entrez Gene: 24236 Rat
- Omim: 120831 Human
- SwissProt: P20851 Human
- SwissProt: Q63515 Rat
- Unigene: 99886 Human
- Unigene: 11151 Rat
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Alternative names
- C4b binding protein beta chain antibody
- C4b-binding protein beta chain antibody
- C4BP antibody
see all
Images
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All lanes : Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430) at 1/1000 dilution
Lane 1 : Human plasma
Lane 2 : Human ovary cancer extract.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID: 2300577)
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Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430) at 1/10000 dilution + Human serum at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID: 2300577)
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Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430) at 1/1000 dilution + Rat plasma at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID: 2300577)
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Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Plasma staining on Human bladder carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat liver tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling C4 binding protein/C4BPB with ab199430 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasm staining on HepG2 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab199430 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
C4 binding protein/C4BPB was immunoprecipitated from 1mg of Human serum with ab199430 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab199430 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human serum 10 µg. Lane 2: Human serum following immunoprecipitation. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199430 in Human serum.
Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure: 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab199430 has been referenced in 1 publication.
- Kobayashi S et al. Collagen type I-mediated mechanotransduction controls epithelial cell fate conversion during intestinal inflammation. Inflamm Regen 42:49 (2022). PubMed: 36443773