Overview

  • Product name

    Anti-C4a antibody [C4D204]
    See all C4a primary antibodies
  • Description

    Mouse monoclonal [C4D204] to C4a
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human C4A. Recombinant Human Complement 4d protein.
    Database link: P0C0L4

  • Positive control

    • kidney Transplant Tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Bioreactor concentrate
  • Clonality

    Monoclonal
  • Clone number

    C4D204
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/100 - 1/200.
ICC/IF 1/50 - 1/100.

Target

  • Function

    C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Involvement in disease

    Defects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.
  • Sequence similarities

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications

    Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acidic complement C4 antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 2 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
    • C4a anaphylatoxin antibody
    • C4A antibody
    • C4AD antibody
    • CO4A_HUMAN antibody
    • Complement C4 A antibody
    • Complement C4 gamma chain antibody
    • Complement component 4A antibody
    • Complement component 4A preproprotein antibody
    • CPAMD2 antibody
    • RG antibody
    • Rodgers form of C4 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded kidney transplant tissue  labeling C4A with ab187278 at 1/100 dilution for 30 minutes at RT.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded kidney transplant tissue labeling C4A with ab187278 at 1/100 dilution for 30 minutes at RT.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded kidney transplant tissue labeling C4A with ab187278 at 1/100 dilution for 30 minutes at RT.

References

ab187278 has not yet been referenced specifically in any publications.

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