RecombinantRabMAb

Recombinant Anti-C4b antibody [EPR11203-27] (ab181241)

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Western blot - Anti-C4b antibody [EPR11203-27] (ab181241)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4b antibody [EPR11203-27] (ab181241)
  • Western blot - Anti-C4b antibody [EPR11203-27] (ab181241)
  • Immunocytochemistry - Anti-C4b antibody [EPR11203-27] (ab181241)
  • Anti-C4b antibody [EPR11203-27] (ab181241)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11203-27] to C4b
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-C4b antibody [EPR11203-27]
    See all C4b primary antibodies

  • Description

    Rabbit monoclonal [EPR11203-27] to C4b

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 96% homology with C4a. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human C4b aa 1100-1350. The exact sequence is proprietary.
    Database link: P0C0L5

  • Positive control

    • ICC: HepG2 cells; IHC: Human liver tissue; WB: HepG2 whole cell lysate. Human plasma and serum lysates. Human liver, heart and testis tissue lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11203-27

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100.

For unpurified use at 1/500

 
WB 1/10000. Detects a band of approximately 90,190 kDa (predicted molecular weight: 193 kDa).

For unpurified use at 1/1000-1/2000 dilution
IHC-P 1/75. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/250

Target

  • Function

    C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

  • Sequence similarities

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.

  • Post-translational
    modifications

    Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Basic complement C4 antibody
    • C2B5 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
    • C4b antibody
    • C4B1 antibody
    • C4B2 antibody
    • C4B3 antibody
    • CO4B_HUMAN antibody
    • Complement C4 gamma chain antibody
    • Complement component 4B antibody
    • CPAMD3 antibody
    see all

Images

  • Western blot - Anti-C4b antibody [EPR11203-27] (ab181241)
    Western blot - Anti-C4b antibody [EPR11203-27] (ab181241)
    All lanes : Anti-C4b antibody [EPR11203-27] (ab181241) at 1/10000 dilution (purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg
    Lane 2 : Human plasma lysate at 15 µg
    Lane 3 : Human serum lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 193 kDa
    Observed band size: 200,70,93 kDa
    why is the actual band size different from the predicted?



    The molecular weights observed are consistent with what have been described in the literature (PMID: 15632414, 31178241 and 30210498).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4b antibody [EPR11203-27] (ab181241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4b antibody [EPR11203-27] (ab181241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling C4b with purified ab181241 at 1/75 dilution (9.53 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-C4b antibody [EPR11203-27] (ab181241)
    Western blot - Anti-C4b antibody [EPR11203-27] (ab181241)
    All lanes : Anti-C4b antibody [EPR11203-27] (ab181241) at 1/10000 dilution (purified)

    Lane 1 : Human liver lysate
    Lane 2 : Human heart lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 193 kDa
    Observed band size: 200,70,93 kDa why is the actual band size different from the predicted?



    The molecular weights observed are consistent with what have been described in the literature (PMID: 15632414, 31178241 and 30210498).

  • Immunocytochemistry - Anti-C4b antibody [EPR11203-27] (ab181241)
    Immunocytochemistry - Anti-C4b antibody [EPR11203-27] (ab181241)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling C4b with purified ab181241 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Anti-C4b antibody [EPR11203-27] (ab181241)
    Anti-C4b antibody [EPR11203-27] (ab181241)

References (1)

Publishing research using ab181241? Please let us know so that we can cite the reference in this datasheet.

ab181241 has been referenced in 1 publication.

  • Bottermann M  et al. Complement C4 Prevents Viral Infection through Capsid Inactivation. Cell Host Microbe 25:617-629.e7 (2019). PubMed: 30926239

Customer reviews and Q&As

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ELISA abreview for Anti-C4b antibody [EPR11203-27]

 Below Average
Abreviews
Application
ELISA
Sample
Human Serum (Human pooled serum)
Specification
Human pooled serum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Type
Indirect
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Submitted Mar 02 2016

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