Recombinant
RabMAb

Recombinant Anti-C4b antibody [EPR11203-27] (ab181241)

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Overview

  • Product name

    Anti-C4b antibody [EPR11203-27]
    See all C4b primary antibodies

  • Description

    Rabbit monoclonal [EPR11203-27] to C4b

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 96% homology with C4a. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human C4b aa 1100-1350. The exact sequence is proprietary.
    Database link: P0C0L5

  • Positive control

    • HepG2 cell lysate; Human fetal liver lysate; Human liver and hepatocellular carcinoma tissues; HepG2 cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11203-27

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 90,190 kDa (predicted molecular weight: 193 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.

Target

  • Function

    C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

  • Sequence similarities

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.

  • Post-translational
    modifications

    Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Basic complement C4 antibody
    • C2B5 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
    • C4b antibody
    • C4B1 antibody
    • C4B2 antibody
    • C4B3 antibody
    • CO4B_HUMAN antibody
    • Complement C4 gamma chain antibody
    • Complement component 4B antibody
    • CPAMD3 antibody
    see all

Images

  • Western blot - Anti-C4b antibody [EPR11203-27] (ab181241)
    Western blot - Anti-C4b antibody [EPR11203-27] (ab181241)
    All lanes : Anti-C4b antibody [EPR11203-27] (ab181241) at 1/2000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 193 kDa
    Observed band size: 190,90 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4b antibody [EPR11203-27] (ab181241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4b antibody [EPR11203-27] (ab181241)

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular carcinoma tissue labeling C4b with ab181241 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4b antibody [EPR11203-27] (ab181241)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4b antibody [EPR11203-27] (ab181241)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling C4b with ab181241 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-C4b antibody [EPR11203-27] (ab181241)
    Immunocytochemistry/ Immunofluorescence - Anti-C4b antibody [EPR11203-27] (ab181241)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling C4b with ab181241 at 1/500 dilution. followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

References

This product has been referenced in:

See 1 Publication for this product

Publishing research using ab181241? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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ELISA abreview for Anti-C4b antibody [EPR11203-27]

 Below Average
Abreviews
Application
ELISA
Sample
Human Serum (Human pooled serum)
Specification
Human pooled serum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Type
Indirect
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Verified customer

Submitted Mar 02 2016

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