Anti-C4b antibody [EPR11203-27] (ab181241)
- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-C4b antibody [EPR11203-27]
See all C4b primary antibodies -
DescriptionRabbit monoclonal [EPR11203-27] to C4b
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
-
Species reactivityReacts with: Human
-
Immunogen
Recombinant fragment within Human C4b aa 1100-1350. The exact sequence is proprietary.
Database link: P0C0L5 -
Positive control
- HepG2 cell lysate; Human fetal liver lysate; Human liver and hepatocellular carcinoma tissues; HepG2 cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR11203-27
-
IsotypeIgG
-
Research areas
Associated products
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab181241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 90,190 kDa (predicted molecular weight: 193 kDa). | |
| IHC-P | 1/250. | |
| ICC/IF | 1/500. |
Target
-
FunctionC4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. -
Sequence similaritiesContains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
Post-translational
modificationsPrior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase. -
Cellular localizationSecreted.
- Information by UniProt
-
Database links
- Entrez Gene: 100293534 Human
- Entrez Gene: 100507685 Human
- Entrez Gene: 720 Human
- Entrez Gene: 721 Human
- Omim: 120820 Human
- SwissProt: P0C0L4 Human
- SwissProt: P0C0L5 Human
- Unigene: 534847 Human
see all -
Alternative names
- Basic complement C4 antibody
- C2B5 antibody
- C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
see all
Images
-
![Western blot - Anti-C4b antibody [EPR11203-27] (ab181241)](https://www.abcam.com/ps/products/181/ab181241/Images/ab181241-215154-ab181241WB.jpg)
Western blot - Anti-C4b antibody [EPR11203-27] (ab181241)All lanes : Anti-C4b antibody [EPR11203-27] (ab181241) at 1/2000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 193 kDa
Observed band size: 190,90 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4b antibody [EPR11203-27] (ab181241)](https://www.abcam.com/ps/products/181/ab181241/Images/ab181241-215156-ab181241IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4b antibody [EPR11203-27] (ab181241)Immunohistochemical analysis of paraffin-embedded Human Hepatocellular carcinoma tissue labeling C4b with ab181241 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4b antibody [EPR11203-27] (ab181241)](https://www.abcam.com/ps/products/181/ab181241/Images/ab181241-215155-ab181241IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4b antibody [EPR11203-27] (ab181241)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling C4b with ab181241 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
-
![Immunocytochemistry/ Immunofluorescence - Anti-C4b antibody [EPR11203-27] (ab181241)](https://www.abcam.com/ps/products/181/ab181241/Images/ab181241-215157-ab181241IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-C4b antibody [EPR11203-27] (ab181241)Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling C4b with ab181241 at 1/500 dilution. followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Protocols
References
ab181241 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"