Recombinant Anti-C4b antibody [EPR11203] - BSA and Azide free (ab249454)
![Western blot - Anti-C4b antibody [EPR11203] - BSA and Azide free (ab249454)](https://www.abcam.com/ps/products/249/ab249454/Images/ab249454-402787-anti-c4b-antibody-epr11203-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-C4b antibody [EPR11203] - BSA and Azide free (ab249454)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11203] to C4b - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-C4b antibody [EPR11203] - BSA and Azide free
See all C4b primary antibodies -
Description
Rabbit monoclonal [EPR11203] to C4b - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
-
Immunogen
Recombinant fragment within Human C4b. The exact sequence is proprietary.
Database link: P0C0L5 -
General notes
Ab249454 is the carrier-free version of ab168358. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab249454 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11203 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 193 kDa.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 193 kDa. |
|
Flow Cyt
Use at an assay dependent concentration. |
Target
-
Function
C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. -
Sequence similarities
Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
Post-translational
modificationsPrior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 100293534 Human
- Entrez Gene: 100507685 Human
- Entrez Gene: 720 Human
- Entrez Gene: 721 Human
- Omim: 120820 Human
- SwissProt: P0C0L4 Human
- SwissProt: P0C0L5 Human
- Unigene: 534847 Human
see all -
Alternative names
- Basic complement C4 antibody
- C2B5 antibody
- C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
see all
Images
-
Western blot - Anti-C4b antibody [EPR11203] - BSA and Azide free (ab249454)All lanes : Anti-C4b antibody [EPR11203] (ab168358) at 1/1000 dilution
Lane 1 : Human serum lysate
Lane 2 : Human fetal liver lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 193 kDaThis data was developed using ab168358, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-C4b antibody [EPR11203] - BSA and Azide free (ab249454)](https://www.abcam.com/ps/products/249/ab249454/Images/ab249454-1-anti-c4b-antibody-epr11203-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-C4b antibody [EPR11203] - BSA and Azide free (ab249454) -
![Anti-C4b antibody [EPR11203] - BSA and Azide free (ab249454)](https://www.abcam.com/ps/products/249/ab249454/Images/ab249454-3-benefits-of-recombinant-antibodies.png)
Anti-C4b antibody [EPR11203] - BSA and Azide free (ab249454)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab249454 has not yet been referenced specifically in any publications.
