Overview

  • Product name

  • Description

    Rabbit polyclonal to C4BPA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to an internal region between amino acids 272-413 of the Human C4BPA protein (P04003).

  • Positive control

    • Human blood vessel tissue. Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
WB 1/250 - 1/500.

Target

  • Function

    Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.
  • Tissue specificity

    Chylomicrons in the plasma.
  • Sequence similarities

    Contains 8 Sushi (CCP/SCR) domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C4b-binding protein alpha chain antibody
    • C4bp antibody
    • C4bpa antibody
    • C4BPA_HUMAN antibody
    • Complement component 4 binding protein alpha antibody
    • Complement component 4 binding protein antibody
    • Proline-rich protein antibody
    • PrP antibody
    • RP11-164O23.4 antibody
    see all

Images

  • All lanes : Anti-C4BPA antibody (ab121141) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)

    Lane 2 : Over-expression Lysate [Co-expressed with a C-terminal proprietary tag (3.1 KDa)] in mammalian HEK293T cells


    Molecular Marker shown at [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
  • Immunohistochemical staining of Human blood vessel with ab121141 at 1/250 dilution, showing distinct positivity in blood plasma.

References

ab121141 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121141.
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