Key features and details
- Rabbit polyclonal to C4BPA
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C4BPA antibody
See all C4BPA primary antibodies
DescriptionRabbit polyclonal to C4BPA
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to an internal region between amino acids 272-413 of the Human C4BPA protein (P04003).
- Human blood vessel tissue. Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionControls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.
Tissue specificityChylomicrons in the plasma.
Sequence similaritiesContains 8 Sushi (CCP/SCR) domains.
- Information by UniProt
- C4b-binding protein alpha chain antibody
- C4bp antibody
- C4bpa antibody
All lanes : Anti-C4BPA antibody (ab121141) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate [Co-expressed with a C-terminal proprietary tag (3.1 KDa)] in mammalian HEK293T cells
Molecular Marker shown at [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Immunohistochemical staining of Human blood vessel with ab121141 at 1/250 dilution, showing distinct positivity in blood plasma.
ab121141 has not yet been referenced specifically in any publications.