Key features and details
- Rabbit polyclonal to C4BPA - C-terminal
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C4BPA antibody - C-terminal
See all C4BPA primary antibodies
DescriptionRabbit polyclonal to C4BPA - C-terminal
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human C4BPA aa 470-499 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P04003
- IHC-P: Human liver tissue. WB: WiDr cell lysate. Flow Cytometry: WiDr cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 67 kDa.|
FunctionControls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.
Tissue specificityChylomicrons in the plasma.
Sequence similaritiesContains 8 Sushi (CCP/SCR) domains.
- Information by UniProt
- C4b-binding protein alpha chain antibody
- C4bp antibody
- C4bpa antibody
Formalin-fixed, paraffin-embedded human liver tissue stained for C4BPA with ab230525 at a 1:100 dilution in immunohistochemical analysis.
Anti-C4BPA antibody - C-terminal (ab230525) at 1/1000 dilution + WiDr cell lysates at 35 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Flow Cytometry analysis of WiDr cells, labeling C4BPA with ab230525 at a 1/10 dilution (green) compared to a negative control (black). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230525 has not yet been referenced specifically in any publications.