Overview

  • Product name

    Anti-C4BPA antibody [EPR14780]
    See all C4BPA primary antibodies
  • Description

    Rabbit monoclonal [EPR14780] to C4BPA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human C4BPA aa 50-150. The exact sequence is proprietary.
    Database link: P04003

  • Positive control

    • WB: Human plasma lysate. IHC-P: Human transitional cell carcinoma of bladder tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14780
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab200345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.
  • Tissue specificity

    Chylomicrons in the plasma.
  • Sequence similarities

    Contains 8 Sushi (CCP/SCR) domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C4b-binding protein alpha chain antibody
    • C4bp antibody
    • C4bpa antibody
    • C4BPA_HUMAN antibody
    • Complement component 4 binding protein alpha antibody
    • Complement component 4 binding protein antibody
    • Proline-rich protein antibody
    • PrP antibody
    • RP11-164O23.4 antibody
    see all

Images

  • Anti-C4BPA antibody [EPR14780] (ab200345) at 1/5000 dilution + human plasma lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 30 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • All lanes : Anti-C4BPA antibody [EPR14780] (ab200345) at 1/5000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetalkidney lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 30 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling C4BPA using ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab200345 and secondary antibody only.
    Note: Plasma staining on human transitional cell carcinoma of bladder tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle  tissue labeling C4BPA using ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab200345 and secondary antibody only.
    Note: No staining on human skeletal muscle tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab200345 has not yet been referenced specifically in any publications.

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