Recombinant
RabMAb

Recombinant Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140)

Overview

  • Product name

    Anti-C4BPA antibody [EPR14781(B)] - C-terminal
    See all C4BPA primary antibodies
  • Description

    Rabbit monoclonal [EPR14781(B)] to C4BPA - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human C4BPA aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: P04003

  • Positive control

    • Human plasma lysate. Human fetal liver lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14781(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
IP 1/60.

Target

  • Function

    Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.
  • Tissue specificity

    Chylomicrons in the plasma.
  • Sequence similarities

    Contains 8 Sushi (CCP/SCR) domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • C4b-binding protein alpha chain antibody
    • C4bp antibody
    • C4bpa antibody
    • C4BPA_HUMAN antibody
    • Complement component 4 binding protein alpha antibody
    • Complement component 4 binding protein antibody
    • Proline-rich protein antibody
    • PrP antibody
    • RP11-164O23.4 antibody
    see all

Images

  • Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140) at 1/10000 dilution + Human plasma lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140) at 1/2000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 67 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?

  • Western blot analysis of C4BPA in Human plasma immunoprecipitated with ab182140 at 1/60 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.

References

ab182140 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab182140.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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