Key features and details
- Rabbit polyclonal to C4d
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-C4d antibody
See all C4d primary antibodies
DescriptionRabbit polyclonal to C4d
SpecificityRecognizes C4d-A and C4d-B.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservatives: 0.09% Sodium azide, 0.1% CMIT/MIT 3:1
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab36075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/80. Antigen retrieval with a citrate buffer system (or equivalent) is recommended.|
FunctionC4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
Involvement in diseaseDefects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.
Sequence similaritiesContains 1 anaphylatoxin-like domain.
Contains 1 NTR domain.
modificationsPrior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
- Information by UniProt
- acidic C4 antibody
- Acidic complement C4 antibody
- basic C4 antibody
Immunohostochemical analysis of C4d expression in paraffin embedded formalin fixed human glomerular mesangial tissue, using 1/40 ab36075. Staining developed using high sensitivity Avidin/Biotin detection system.
ab36075 staining C4d in human kidney cells by Immunocytochemistry/ Immunofluorescence (snap frozen). The cells were acetone fixed. Samples were incubated with primary antibody at 1/60 for 1 hour at 25°C. The secondary antibody used was an IgG conjugated to Alexa Fluor® 488.
Ab36075 staining C4d in Human kidney tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin embedded sections). Tissue was fixed with formaldehyde and heat mediated antigen retrieval was performed at 100°C with citrate for 20 minutes. Primary antibody was incubated at 1/80 dilution for 20 minutes at 25°C. A HRP conjugated anti-mouse IgG was used as a secondary antibody.
Ab36075 staining C4d in Human nasal sinus tissue by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 20% milk for 20 minutes. Primary antibody was incubated at a 1/80 dilution in PBS for 1 hour at 25°C. A biotin conjugated goat anti-rabbit was used as a secondary antibody at 1/100 dilution.
ab36075 has been referenced in 5 publications.
- Zhu X et al. All-trans retinoic acid protects mesenchymal stem cells from immune thrombocytopenia by regulating the complement-IL-1ß loop. Haematologica N/A:N/A (2019). PubMed: 30679324
- Sorbara MT et al. Complement C3 Drives Autophagy-Dependent Restriction of Cyto-invasive Bacteria. Cell Host Microbe 23:644-652.e5 (2018). PubMed: 29746835
- Jiang Y et al. The Effect of Histological CD20-Positive B Cell Infiltration in Acute Cellular Rejection on Kidney Transplant Allograft Survival. J Immunol Res 2016:7473239 (2016). PubMed: 28058267
- Almarastani M et al. Salvaging of severely ruptured living-related renal allograft secondary to acute antibody mediated rejection. Int J Surg Case Rep 5:723-6 (2014). IHC-P ; Human . PubMed: 25216193
- Sansonno D et al. Role of the receptor for the globular domain of c1q protein in the pathogenesis of hepatitis C virus-related cryoglobulin vascular damage. J Immunol 183:6013-20 (2009). IHC-P ; Human . PubMed: 19828637