• Product name
  • Description
    Rabbit polyclonal to C4d
  • Host species
  • Specificity
    Recognizes C4d-A and C4d-B.
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human C4d aa 1223-1237 (C terminal) conjugated to keyhole limpet haemocyanin. Region 100% conserved in C4d-B (Uniprot: P0C0L5)


    Database link: P0C0L4

  • Positive control
    • Glomerular mesangial tissue


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab36075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/80. Antigen retrieval with a citrate buffer system (or equivalent) is recommended.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/20 - 1/80.
IHC-Fr 1/20 - 1/80.


  • Function
    C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Involvement in disease
    Defects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.
  • Sequence similarities
    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • acidic C4 antibody
    • Acidic complement C4 antibody
    • basic C4 antibody
    • basic complement C4 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
    • C4 antibody
    • C4, chido form antibody
    • C4, Rodgers form antibody
    • C4-1 antibody
    • C4a anaphylatoxin antibody
    • C4A antibody
    • C4A2 antibody
    • C4A3 antibody
    • C4A4 antibody
    • C4A6 antibody
    • C4AD antibody
    • C4b antibody
    • C4B_2 antibody
    • C4B1 antibody
    • C4B12 antibody
    • C4B2 antibody
    • C4B3 antibody
    • C4B5 antibody
    • C4BD antibody
    • C4d antibody
    • C4d fragment antibody
    • C4F antibody
    • C4S antibody
    • CH antibody
    • Chido form of C4 antibody
    • CO4 antibody
    • CO4A_HUMAN antibody
    • Complement C4 gamma chain antibody
    • complement C4-A antibody
    • complement C4-B antibody
    • complement C4-B-like antibody
    • complement C4B1a antibody
    • Complement component 4A antibody
    • complement component 4A (Rodgers blood group) antibody
    • Complement component 4B antibody
    • complement component 4B (Chido blood group) antibody
    • complement component 4B (Chido blood group), copy 2 antibody
    • complement component 4B (Chido blood group)-like antibody
    • Complement component 4F antibody
    • Complement component 4S antibody
    • CPAMD2 antibody
    • CPAMD3 antibody
    • DADB-112B14.11 antibody
    • MGC164979 antibody
    • RG antibody
    • Rodgers form of C4 antibody
    see all


  • Immunohostochemical analysis of C4d expression in paraffin embedded formalin fixed human glomerular mesangial tissue, using 1/40 ab36075. Staining developed using high sensitivity Avidin/Biotin detection system.
  • ab36075 staining C4d in human kidney cells by Immunocytochemistry/ Immunofluorescence (snap frozen). The cells were acetone fixed. Samples were incubated with primary antibody at 1/60 for 1 hour at 25°C. The secondary antibody used was an IgG conjugated to Alexa Fluor® 488.
  • Ab36075 staining C4d in Human kidney tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin embedded sections). Tissue was fixed with formaldehyde and heat mediated antigen retrieval was performed at 100°C with citrate for 20 minutes. Primary antibody was incubated at 1/80 dilution for 20 minutes at 25°C. A HRP conjugated anti-mouse IgG was used as a secondary antibody.

    See Abreview

  • Ab36075 staining C4d in Human nasal sinus tissue by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 20% milk for 20 minutes. Primary antibody was incubated at a 1/80 dilution in PBS for 1 hour at 25°C. A biotin conjugated goat anti-rabbit was used as a secondary antibody at 1/100 dilution.

    See Abreview


This product has been referenced in:
  • Almarastani M  et al. Salvaging of severely ruptured living-related renal allograft secondary to acute antibody mediated rejection. Int J Surg Case Rep 5:723-6 (2014). IHC-P ; Human . Read more (PubMed: 25216193) »
  • Sansonno D  et al. Role of the receptor for the globular domain of c1q protein in the pathogenesis of hepatitis C virus-related cryoglobulin vascular damage. J Immunol 183:6013-20 (2009). IHC-P ; Human . Read more (PubMed: 19828637) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for the reply.

Could you please confirm whether either of the vials of ab36075 purchased showed any staining (one of the 20xxxx and the one from the xxxxx)?

Also, I asked for some feedback of how the antibodies had been stor...

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Thank you for contacting us. The antibody is supplied in liquid form and does not have to be reconstituted. The vial should contain 50 ul (0.2 mg/ml), if you do not see it you should spin it down. I can direct you to a starting dilution if you can tell...

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Thank you for your reply.

Having reviewed this case it seems that using the protocol the customer has applied the antibody should be producing staining.

May I ask, how has the antibody been stored? At what temperature and was it ali...

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Thank you very much for having followed up with the customer and provided this additional information.

When looking through the protocol, I was surprised about the information on the secondary antibody. The customer mentioned to have used a r...

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We have previously been in contact regarding ab36075, which was not providing satisfactory results.

A free of charge replacement vial was issued and I am writing to inquire if the replacement product has worked. I would appreciate if you could...

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Thank you for contacting us.

Your credit note ID is XXXXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used...

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Thank you for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I ap...

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Thank you for this additional information.

I would be pleased to investigate this issue further to see whether we could provide some protocol tips. Can you please therefore send us the protocol details with help of the filled questionnaire (i...

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Thank you for your response.

I am sorry to hear that you have been experiencing problems when using our products. Our anti-Twist antibody in guaranteed to work in mouse tissues in IHC-Fr. We have added recommended dilutions or specific proto...

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Thank you for contacting us.

I am sorry to hearthat a customer is experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
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