Anti-C4d antibody (ab36075)
Key features and details
- Rabbit polyclonal to C4d
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C4d antibody
See all C4d primary antibodies -
Description
Rabbit polyclonal to C4d -
Host species
Rabbit -
Specificity
Recognizes C4d-A and C4d-B. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human C4d aa 1223-1237 (C terminal) conjugated to keyhole limpet haemocyanin. Region 100% conserved in C4d-B (Uniprot: P0C0L5)
Sequence:ETGDNLYWGSVTGSQ
Database link: P0C0L4 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituents: 99% PBS, 0.1% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
1/20 - 1/80. Antigen retrieval with a citrate buffer system (or equivalent) is recommended.
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Notes |
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IHC-P
1/20 - 1/80. Antigen retrieval with a citrate buffer system (or equivalent) is recommended. |
Target
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Function
C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. -
Involvement in disease
Defects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis. -
Sequence similarities
Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
Post-translational
modificationsPrior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 100293534 Human
- Entrez Gene: 100507685 Human
- Entrez Gene: 720 Human
- Entrez Gene: 721 Human
- Omim: 120810 Human
- Omim: 120820 Human
- SwissProt: P0C0L4 Human
- SwissProt: P0C0L5 Human
see all -
Alternative names
- acidic C4 antibody
- Acidic complement C4 antibody
- basic C4 antibody
see all
Images
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Immunohostochemical analysis of C4d expression in paraffin embedded formalin fixed human glomerular mesangial tissue, using 1/40 ab36075. Staining developed using high sensitivity Avidin/Biotin detection system.
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Ab36075 staining C4d in Human kidney tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin embedded sections). Tissue was fixed with formaldehyde and heat mediated antigen retrieval was performed at 100°C with citrate for 20 minutes. Primary antibody was incubated at 1/80 dilution for 20 minutes at 25°C. A HRP conjugated anti-mouse IgG was used as a secondary antibody.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab36075 has been referenced in 6 publications.
- Fukami Y et al. Role of complement components in vasculitic neuropathy associated with systemic lupus erythematosus and rheumatoid arthritis. Muscle Nerve 66:175-182 (2022). PubMed: 35581952
- Zhu X et al. All-trans retinoic acid protects mesenchymal stem cells from immune thrombocytopenia by regulating the complement-IL-1ß loop. Haematologica N/A:N/A (2019). PubMed: 30679324
- Sorbara MT et al. Complement C3 Drives Autophagy-Dependent Restriction of Cyto-invasive Bacteria. Cell Host Microbe 23:644-652.e5 (2018). PubMed: 29746835
- Jiang Y et al. The Effect of Histological CD20-Positive B Cell Infiltration in Acute Cellular Rejection on Kidney Transplant Allograft Survival. J Immunol Res 2016:7473239 (2016). PubMed: 28058267
- Almarastani M et al. Salvaging of severely ruptured living-related renal allograft secondary to acute antibody mediated rejection. Int J Surg Case Rep 5:723-6 (2014). IHC-P ; Human . PubMed: 25216193
- Sansonno D et al. Role of the receptor for the globular domain of c1q protein in the pathogenesis of hepatitis C virus-related cryoglobulin vascular damage. J Immunol 183:6013-20 (2009). IHC-P ; Human . PubMed: 19828637